The frequency and consequences of multipolar mitoses in undifferentiated embryonic stem cells
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68081707%3A_____%2F19%3A00522155" target="_blank" >RIV/68081707:_____/19:00522155 - isvavai.cz</a>
Alternative codes found
RIV/00216224:14110/19:00108159 RIV/00159816:_____/19:00072511
Result on the web
<a href="http://jab.zsf.jcu.cz/doi/10.32725/jab.2019.018.pdf" target="_blank" >http://jab.zsf.jcu.cz/doi/10.32725/jab.2019.018.pdf</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.32725/jab.2019.018" target="_blank" >10.32725/jab.2019.018</a>
Alternative languages
Result language
angličtina
Original language name
The frequency and consequences of multipolar mitoses in undifferentiated embryonic stem cells
Original language description
Embryonic stem (ES) cells are pluripotent cells widely used in cell therapy and tissue engineering. However, the broader clinical applications of ES cells are limited by their genomic instability and karyotypic abnormalities. Thus, understanding the mechanisms underlying ES cell karyotypic abnormalities is critical to optimizing their dinical use. In this study, we focused on proliferating human and mouse ES cells undergoing multipolar divisions. Specifically, we analyzed the frequency and outcomes of such divisions using a combination of time-lapse microscopy and cell tracking. This revealed that cells resulting from multipolar divisions were not only viable, but they also frequently underwent subsequent cell divisions. Our novel data also showed that in human and mouse ES cells, multipolar spindles allowed more robust escape from chromosome segregation control mechanisms than bipolar spindles. Considering the frequency of multipolar divisions in proliferating ES cells, it is conceivable that cell division errors underlie ES cell karyotypic instability.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
30104 - Pharmacology and pharmacy
Result continuities
Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2019
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Journal of Applied Biomedicine
ISSN
1214-021X
e-ISSN
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Volume of the periodical
17
Issue of the periodical within the volume
4
Country of publishing house
CZ - CZECH REPUBLIC
Number of pages
9
Pages from-to
209-217
UT code for WoS article
000510179600002
EID of the result in the Scopus database
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