OECT MULTIELECTRODE ARRAY FOR REAL-TIME CELL CULTURE MONITORING

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68081707%3A_____%2F19%3A00523258" target="_blank" >RIV/68081707:_____/19:00523258 - isvavai.cz</a>

Alternative codes found

RIV/00216305:26310/19:PU136047

Result on the web

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DOI - Digital Object Identifier

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Result language

angličtina

Original language name

OECT MULTIELECTRODE ARRAY FOR REAL-TIME CELL CULTURE MONITORING

Original language description

The significant cost of newly developed pharmacological products is due to the toxicity examination performed on animals in pre-clinical trials. Our ultimate goal is to develop a biosensor array to determine acute cardiotoxicity. It capitalizes on the fact that heart muscle cells (cardiomyocytes) show beating in vitro. Based on the beating characteristics their pathophysiological status can be deducted. In addition to microscopic imaging, the method of monitoring the action and field potential of synchronously beating cardiomyocyte syncytia on multielectrode arrays (MEA) appears to be a suitable method for the investigation of the condition of cardiac cells. For this purpose, MEA on the basis of organic electrochemical transistors (OECTs) has been developed. The organic polymer PEDOT:PSS was utilized to meet the strict surface biocompatibility demand for a surface in contact with media and living cardiac cells. The MEA consisted of a microplate of a 12x8 chimney well array with transistors on the bottom for cell cultivation. For the fabrication (a screen printing method) primarily a contact field and an organic polymer was used. The device was tested in a simulation mode for the response of the drain current to the voltage pulses applied to the gate electrode up to 100 mu V. The response to such gate voltage renders the device sensitive enough to detect the electrical signal of beating cardiomyocytes. Further development is directed towards higher time resolution by means of miniaturization of the transistors' channel.

Czech name

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Czech description

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Type

D - Article in proceedings

CEP classification

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OECD FORD branch

21001 - Nano-materials (production and properties)

Project

Result was created during the realization of more than one project. More information in the Projects tab.

Continuities

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2019

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Article name in the collection

10TH ANNIVERSARY INTERNATIONAL CONFERENCE ON NANOMATERIALS RESEARCH & APPLICATION (NANOCON 2018 (R))

ISBN

978-80-87294-89-5

ISSN

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e-ISSN

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Number of pages

6

Pages from-to

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Publisher name

TANGER Ltd

Place of publication

Ostrava

Event location

Brno

Event date

Oct 17, 2018

Type of event by nationality

WRD - Celosvětová akce

UT code for WoS article

000513131900058