Simultaneous voltammetric determination of free tryptophan, uric acid, xanthine and hypoxanthine in plasma and urine
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68081707%3A_____%2F20%3A00521718" target="_blank" >RIV/68081707:_____/20:00521718 - isvavai.cz</a>
Alternative codes found
RIV/00216305:26620/20:PU140080
Result on the web
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DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1016/j.electacta.2019.135132" target="_blank" >10.1016/j.electacta.2019.135132</a>
Alternative languages
Result language
angličtina
Original language name
Simultaneous voltammetric determination of free tryptophan, uric acid, xanthine and hypoxanthine in plasma and urine
Original language description
We have designed an improved electrochemical analysis of clinical samples in bodily fluids. The method allows reliable identification of several coexisting substances (tryptophan, uric acid, xanthine and hypoxanthine) present on different physiological levels. We prepared in situ electrogenerated graphite oxides (GrO) directly on the surface of a graphite pencil electrode. GrO presence enhances electrooxidation signals of small aromatic molecules and can shift their oxidation potentials. The oxidation potentials of tryptophan and tyrosine in their free and protein-bound forms, and free xanthine are very close to each other on common carbon-based materials. We found that free tryptophan's oxidation signal is significantly more enhanced (about 11-times) than the free tyrosine signal (only negligibly in the given conditions). Distinguishing xanthine's oxidation signal from that of the free tryptophan is allowed by their approximately 60 mV separation, sufficient for parallel determination by signal deconvolution. Additionally, we show that proteins containing tyrosine and/or tryptophan behave as electro-inactive and do not interfere with free amino acid determination on the GrO-modified graphite electrodes, while they strongly influence all species' detection on other carbon-based materials. Determining all individual compounds was possible at corresponding physiological values after sole dilution of human plasma (urine) and direct measurement with no pre-separation steps. (C) 2019 Elsevier Ltd. All rights reserved.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10405 - Electrochemistry (dry cells, batteries, fuel cells, corrosion metals, electrolysis)
Result continuities
Project
<a href="/en/project/GA18-18154S" target="_blank" >GA18-18154S: New tools of label-free electrochemical analysis of protein interactions with nucleic acids and proteins</a><br>
Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2020
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Electrochimica acta
ISSN
0013-4686
e-ISSN
—
Volume of the periodical
329
Issue of the periodical within the volume
JAN 2020
Country of publishing house
GB - UNITED KINGDOM
Number of pages
10
Pages from-to
135132
UT code for WoS article
000498391900034
EID of the result in the Scopus database
2-s2.0-85074152340