Native and denatured forms of proteins can be discriminated at edge plane carbon electrodes

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68081707%3A_____%2F12%3A00380149" target="_blank" >RIV/68081707:_____/12:00380149 - isvavai.cz</a>
Result on the web
<a href="http://dx.doi.org/10.1016/j.aca.2012.05.012" target="_blank" >http://dx.doi.org/10.1016/j.aca.2012.05.012</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1016/j.aca.2012.05.012" target="_blank" >10.1016/j.aca.2012.05.012</a>

Result language
angličtina
Original language name
Native and denatured forms of proteins can be discriminated at edge plane carbon electrodes
Original language description
In an attempt to develop a label-free electrochemical method for detection of changes in protein structures based on oxidizability of tyrosine and tryptophan residues we tested different types of carbon electrodes. We found that using edge plane pyrolytic graphite electrode we can discriminate between native and denatured forms of human serum albumin and of other proteins. The electrochemical denaturation curve agreed reasonably well with that based on intrinsic fluorescence of tyrosine and tryptophan.It can be expected that the electrochemical method will be applicable to a large number of proteins and may become useful in biomedicine and proteomics.
Czech name
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Czech description
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Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
BO - Biophysics
OECD FORD branch
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Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>Z - Vyzkumny zamer (s odkazem do CEZ)

Publication year
2012
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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