Electroanalysis of unnatural base pair content in plasmid DNA generated in a semi-synthetic organism

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68081707%3A_____%2F20%3A00536183" target="_blank" >RIV/68081707:_____/20:00536183 - isvavai.cz</a>
Alternative codes found
RIV/00216224:14740/20:00114754
Result on the web
<a href="https://www.sciencedirect.com/science/article/pii/S0013468620316911?via%3Dihub" target="_blank" >https://www.sciencedirect.com/science/article/pii/S0013468620316911?via%3Dihub</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1016/j.electacta.2020.137298" target="_blank" >10.1016/j.electacta.2020.137298</a>

Result language
angličtina
Original language name
Electroanalysis of unnatural base pair content in plasmid DNA generated in a semi-synthetic organism
Original language description
While standard Watson-Crick base pairing normally involves hydrogen bonds that join size complemen-tary nitrogenous heterocycles, certain unnatural base pairs (UBP) can support replication, transcription, and translation, including these in semi-synthetic organisms (SSO), using size complementarity alone. In this paper we show that the UBPs can be analyzed electrochemically as components of riboor 2'-deoxyribonucleosides, corresponding (2'-deoxy)triphosphates, as well as incorporated into DNA. Here, performance of the electrochemical methods profits from the ability of the unnatural bases 5SICS and TPT3 adsorbed at the surface of a mercury electrode to catalyze hydrogen evolution in acidic media. This allows semi-quantitative detection of single UBP within plasmid DNA generated in a SSO. This electrochemical approach provides new way for direct, fast, and low cost UBP analysis and can supplement or replace currently used methods of UBP detection. (C) 2020 Elsevier Ltd. All rights reserved.
Czech name
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Czech description
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Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
—
OECD FORD branch
10405 - Electrochemistry (dry cells, batteries, fuel cells, corrosion metals, electrolysis)

Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year
2020
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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