Spatiotemporal Mislocalization of Nuclear Membrane-Associated Proteins in gamma-Irradiation-Induced Senescent Cells

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68081707%3A_____%2F20%3A00540504" target="_blank" >RIV/68081707:_____/20:00540504 - isvavai.cz</a>

Result on the web

<a href="https://www.mdpi.com/2073-4409/9/4/999" target="_blank" >https://www.mdpi.com/2073-4409/9/4/999</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.3390/cells9040999" target="_blank" >10.3390/cells9040999</a>

Result language

angličtina

Original language name

Spatiotemporal Mislocalization of Nuclear Membrane-Associated Proteins in gamma-Irradiation-Induced Senescent Cells

Original language description

Cellular senescence, induced by genotoxic or replication stress, is accompanied by defects in nuclear morphology and nuclear membrane-heterochromatin disruption. In this work, we analyzed cytological and molecular changes in the linker of nucleoskeleton and cytoskeleton (LINC) complex proteins in senescence triggered by gamma-irradiation. We used human mammary carcinoma and osteosarcoma cell lines, both original and shRNA knockdown clones targeting lamin B receptor (LBR) and leading to LBR and lamin B (LB1) reduction. The expression status and integrity of LINC complex proteins (nesprin-1, SUN1, SUN2), lamin A/C, and emerin were analyzed by immunodetection using confocal microscopy and Western blot. The results show frequent mislocalization of these proteins from the nuclear membrane to cytoplasm and micronuclei and, in some cases, their fragmentation and amplification. The timing of these changes clearly preceded the onset of senescence. The LBR deficiency triggered neither senescence nor changes in the LINC protein distribution before irradiation. However, the cytological changes following irradiation were more pronounced in shRNA knockdown cells compared to original cell lines. We conclude that mislocalization of LINC complex proteins is a significant characteristic of cellular senescence phenotypes and may influence complex events at the nuclear membrane, including trafficking and heterochromatin attachment.

Czech name

—

Czech description

—

Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

—

OECD FORD branch

10601 - Cell biology

Project

<a href="/en/project/GC20-04109J" target="_blank" >GC20-04109J: New insights into cooperation of micro- and nano-scale elementary structural chromatin units in decision-making on DNA damage repair pathway (NANOREP)</a><br>

Continuities

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2020

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Cells

ISSN

2073-4409

e-ISSN

—

Volume of the periodical

9

Issue of the periodical within the volume

4

Country of publishing house

CH - SWITZERLAND

Number of pages

19

Pages from-to

999

UT code for WoS article

000535559500210

EID of the result in the Scopus database

2-s2.0-85083871347