Ligand Binding to Dynamically Populated G-Quadruplex DNA
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68081707%3A_____%2F21%3A00541571" target="_blank" >RIV/68081707:_____/21:00541571 - isvavai.cz</a>
Result on the web
<a href="https://chemistry-europe.onlinelibrary.wiley.com/doi/10.1002/cbic.202000792" target="_blank" >https://chemistry-europe.onlinelibrary.wiley.com/doi/10.1002/cbic.202000792</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1002/cbic.202000792" target="_blank" >10.1002/cbic.202000792</a>
Alternative languages
Result language
angličtina
Original language name
Ligand Binding to Dynamically Populated G-Quadruplex DNA
Original language description
Several small-molecule ligands specifically bind and stabilize G-quadruplex (G4) nucleic acid structures, which are considered to be promising therapeutic targets. G4s are polymorphic structures of varying stability, and their formation is dynamic. Here, we investigate the mechanisms of ligand binding to dynamically populated human telomere G4 DNA by using the bisquinolinium based ligand Phen-DC3 and a combination of single-molecule FRET microscopy, ensemble FRET and CD spectroscopies. Different cations are used to tune G4 polymorphism and folding dynamics. We find that ligand binding occurs to pre-folded G4 structures and that Phen-DC3 also induces G4 formation in unfolded single strands. Following ligand binding to dynamically populated G4s, the DNA undergoes pronounced conformational redistributions that do not involve direct ligand-induced G4 conformational interconversion. On the contrary, the redistribution is driven by ligand-induced G4 folding and trapping of dynamically populated short-lived conformation states. Thus, ligand-induced stabilization does not necessarily require the initial presence of stably folded G4s.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10608 - Biochemistry and molecular biology
Result continuities
Project
<a href="/en/project/EF15_003%2F0000477" target="_blank" >EF15_003/0000477: Structural gymnastics of nucleic acids: from molecular principles through biological functions to therapeutic targets.</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Others
Publication year
2021
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Chembiochem
ISSN
1439-4227
e-ISSN
1439-7633
Volume of the periodical
22
Issue of the periodical within the volume
10
Country of publishing house
DE - GERMANY
Number of pages
8
Pages from-to
1811-1817
UT code for WoS article
000625227700001
EID of the result in the Scopus database
2-s2.0-85101924712