Chronopotentiometric Analysis of Single Histones and Histone Octamer at Charged Surfaces
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68081707%3A_____%2F21%3A00546664" target="_blank" >RIV/68081707:_____/21:00546664 - isvavai.cz</a>
Alternative codes found
RIV/00216224:14310/21:00122240
Result on the web
<a href="https://chemistry-europe.onlinelibrary.wiley.com/doi/10.1002/celc.202100817" target="_blank" >https://chemistry-europe.onlinelibrary.wiley.com/doi/10.1002/celc.202100817</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1002/celc.202100817" target="_blank" >10.1002/celc.202100817</a>
Alternative languages
Result language
angličtina
Original language name
Chronopotentiometric Analysis of Single Histones and Histone Octamer at Charged Surfaces
Original language description
Histones play an essential role in the nucleosome structure and organization. The key role of highly positively charged histones is to stabilize negatively charged DNA inside the chromatin. Histones are basic proteins containing a large amount of electroactive residues, namely, arginine and lysine. Additionally, histone H3, on the contrary to other histones, contains cysteine residues. To understand more about the behavior of histones at charged surfaces and the influence of the electroactive amino acid residues content on the catalytic hydrogen evolution reaction, we studied histones using label-free approaches, namely, chronopotentiometric stripping analysis (CPS) and impedance C-d-t curves. While CPS is sensitive to the structure and stability of proteins and their complexes, C-d-t curves reflect changes of the electrical double layer at the charged surface. Application of both label-free methods appears useful for analysis of complex systems. Using a combination of these methods, we could discriminate between individual single core histones, their mixture, and their complex (octamer).
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10405 - Electrochemistry (dry cells, batteries, fuel cells, corrosion metals, electrolysis)
Result continuities
Project
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Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2021
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
ChemElectroChem
ISSN
2196-0216
e-ISSN
2196-0216
Volume of the periodical
8
Issue of the periodical within the volume
17
Country of publishing house
DE - GERMANY
Number of pages
6
Pages from-to
3360-3365
UT code for WoS article
000696501800022
EID of the result in the Scopus database
2-s2.0-85115003541