Fetuin and asialofetuin at charged surfaces: Influence of sialic acid presence

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68081707%3A_____%2F21%3A00549591" target="_blank" >RIV/68081707:_____/21:00549591 - isvavai.cz</a>
Result on the web
<a href="https://www.sciencedirect.com/science/article/pii/S1572665721008274?via%3Dihub" target="_blank" >https://www.sciencedirect.com/science/article/pii/S1572665721008274?via%3Dihub</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1016/j.jelechem.2021.115801" target="_blank" >10.1016/j.jelechem.2021.115801</a>

Result language
angličtina
Original language name
Fetuin and asialofetuin at charged surfaces: Influence of sialic acid presence
Original language description
Major human diseases are associated with changes in glycosylation. The sialic acid is highly expressed saccharide residue typically terminating branches of protein glycans and glycosphingolipids. Here we compared sialoprotein fetuin with its desialylated form asialofetuin using label-free constant current chronopotentiometric stripping (CPS) analysis and impedance C-t curves to better understand the behavior of glycoproteins at charged surfaces. Electrochemistry represents useful tool for study of molecules at charged surfaces. Using CPS peak H, we show that the terminal sialic acids presence in glycans of fetuin stabilizes its molecule against effects of electric field. Additionally the presence of negatively charged sialic acid prevents an interaction between glycoprotein and negatively charged electrode. The different behavior of the glycoproteins according to sialic acid content can also be used for easy determination of sialoglycoproteins.
Czech name
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Czech description
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Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
—
OECD FORD branch
10405 - Electrochemistry (dry cells, batteries, fuel cells, corrosion metals, electrolysis)

Project
<a href="/en/project/GA18-18154S" target="_blank" >GA18-18154S: New tools of label-free electrochemical analysis of protein interactions with nucleic acids and proteins</a><br>
Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year
2021
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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