Localization of METTL16 at the Nuclear Periphery and the Nucleolus Is Cell Cycle-Specific and METTL16 Interacts with Several Nucleolar Proteins

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68081707%3A_____%2F21%3A00553668" target="_blank" >RIV/68081707:_____/21:00553668 - isvavai.cz</a>

Alternative codes found

RIV/00216224:14310/21:00122231

Result on the web

<a href="https://www.mdpi.com/2075-1729/11/7/669/htm" target="_blank" >https://www.mdpi.com/2075-1729/11/7/669/htm</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.3390/life11070669" target="_blank" >10.3390/life11070669</a>

Result language

angličtina

Original language name

Localization of METTL16 at the Nuclear Periphery and the Nucleolus Is Cell Cycle-Specific and METTL16 Interacts with Several Nucleolar Proteins

Original language description

METTL16 methyltransferase is responsible for the methylation of N-6-adenosine (m(6)A) in several RNAs. In mouse cells, we showed that the nuclear distribution of METTL16 is cell cycle-specific. In the G1/S phases, METTL16 accumulates to the nucleolus, while in the G2 phase, the level of METTL16 increases in the nucleoplasm. In metaphase and anaphase, there is a very low pool of the METTL16 protein, but in telophase, residual METTL16 appears to be associated with the newly formed nuclear lamina. In A-type lamin-depleted cells, we observed a reduction of METTL16 when compared with the wild-type counterpart. However, METTL16 does not interact with A-type and B-type lamins, but interacts with Lamin B Receptor (LBR) and Lap2 alpha. Additionally, Lap2 alpha depletion caused METTL16 downregulation in the nuclear pool. Furthermore, METTL16 interacted with DDB2, a key protein of the nucleotide excision repair (NER), and also with nucleolar proteins, including TCOF, NOLC1, and UBF1/2, but not fibrillarin. From this view, the METTL16 protein may also regulate the transcription of ribosomal genes because we observed that the high level of m(6)A in 18S rRNA appeared in cells with upregulated METTL16.

Czech name

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Czech description

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Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

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OECD FORD branch

10606 - Microbiology

Project

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Continuities

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2021

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Life

ISSN

2075-1729

e-ISSN

2075-1729

Volume of the periodical

11

Issue of the periodical within the volume

7

Country of publishing house

CH - SWITZERLAND

Number of pages

16

Pages from-to

669

UT code for WoS article

000676256700001

EID of the result in the Scopus database

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