Expandable Lung Epithelium Differentiated from Human Embryonic Stem Cells
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68081707%3A_____%2F22%3A00561344" target="_blank" >RIV/68081707:_____/22:00561344 - isvavai.cz</a>
Alternative codes found
RIV/00159816:_____/22:00077652 RIV/00209805:_____/22:00079018 RIV/00216224:14110/22:00126034
Result on the web
<a href="https://link.springer.com/article/10.1007/s13770-022-00458-0" target="_blank" >https://link.springer.com/article/10.1007/s13770-022-00458-0</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1007/s13770-022-00458-0" target="_blank" >10.1007/s13770-022-00458-0</a>
Alternative languages
Result language
angličtina
Original language name
Expandable Lung Epithelium Differentiated from Human Embryonic Stem Cells
Original language description
Background The progenitors to lung airway epithelium that are capable of long-term propagation may represent an attractive source of cells for cell-based therapies, disease modeling, toxicity testing, and others. Principally, there are two main options for obtaining lung epithelial progenitors: (i) direct isolation of endogenous progenitors from human lungs and (ii) in vitro differentiation from some other cell type. The prime candidates for the second approach are pluripotent stem cells, which may provide autologous and/or allogeneic cell resource in clinically relevant quality and quantity. Methods By exploiting the differentiation potential of human embryonic stem cells (hESC), here we derived expandable lung epithelium (ELEP) and established culture conditions for their long-term propagation (more than 6 months) in a monolayer culture without a need of 3D culture conditions and/or cell sorting steps, which minimizes potential variability of the outcome. Results These hESC-derived ELEP express NK2 Homeobox 1 (NKX2.1), a marker of early lung epithelial lineage, display properties of cells in early stages of surfactant production and are able to differentiate to cells exhibitting molecular and morphological characteristics of both respiratory epithelium of airway and alveolar regions. Conclusion Expandable lung epithelium thus offer a stable, convenient, easily scalable and high-yielding cell source for applications in biomedicine.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10601 - Cell biology
Result continuities
Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2022
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Jo'jig Gonghag gwa Jaesaeng Uihag
ISSN
1738-2696
e-ISSN
2212-5469
Volume of the periodical
19
Issue of the periodical within the volume
5
Country of publishing house
KR - KOREA, REPUBLIC OF
Number of pages
18
Pages from-to
1033-1050
UT code for WoS article
000807342200001
EID of the result in the Scopus database
2-s2.0-85131534857