Lungosphere Assay: 3D Culture of Lung Epithelial Stem/Progenitor Cells
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00159816%3A_____%2F17%3A00067141" target="_blank" >RIV/00159816:_____/17:00067141 - isvavai.cz</a>
Result on the web
<a href="http://dx.doi.org/10.1007/978-1-4939-7021-6_11" target="_blank" >http://dx.doi.org/10.1007/978-1-4939-7021-6_11</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1007/978-1-4939-7021-6_11" target="_blank" >10.1007/978-1-4939-7021-6_11</a>
Alternative languages
Result language
angličtina
Original language name
Lungosphere Assay: 3D Culture of Lung Epithelial Stem/Progenitor Cells
Original language description
Lung epithelium contains distinctive subpopulations of lung stem/progenitor cells (LSPCs) that are essential for lung epithelial maintenance and repair in vivo. Hence, LSPCs are in the center of interest of lung biology due to their promising therapeutic applications. To reach this goal, proper characterization of LSPCs, understanding of their proliferation and differentiation potentials and elucidation of mechanisms that control them are necessary. Therefore, development of reliable in vitro clonogenic assays has been needed. We established lungosphere assay, an in vitro sphere-forming 3D culture assay that enables to evaluate stem/progenitor cell activity, self-renewal and differentiation capacity of LSPCs and to conveniently test the effect of various treatments on LSPCs. Here we provide a detailed description of procedures for isolation of adult mouse lung epithelial cells, their culture in non-adherent conditions to form LSPC-derived spheroids (lungospheres) and for embedding of lungospheres into 3D extracellular matrix to model processes of lung tissue maintenance in a physiologically relevant microenvironment.
Czech name
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Czech description
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Classification
Type
C - Chapter in a specialist book
CEP classification
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OECD FORD branch
10601 - Cell biology
Result continuities
Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Others
Publication year
2017
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Book/collection name
3D Cell Culture
ISBN
978-1-4939-7019-3
Number of pages of the result
12
Pages from-to
1-12
Number of pages of the book
452
Publisher name
Humana Press
Place of publication
New York
UT code for WoS chapter
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