Lungosphere Assay: 3D Culture of Lung Epithelial Stem/Progenitor Cells

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14110%2F17%3A00095147" target="_blank" >RIV/00216224:14110/17:00095147 - isvavai.cz</a>

Result on the web

<a href="http://dx.doi.org/10.1007/978-1-4939-7021-6_11" target="_blank" >http://dx.doi.org/10.1007/978-1-4939-7021-6_11</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1007/978-1-4939-7021-6_11" target="_blank" >10.1007/978-1-4939-7021-6_11</a>

Result language

angličtina

Original language name

Lungosphere Assay: 3D Culture of Lung Epithelial Stem/Progenitor Cells

Original language description

Lung epithelium contains distinctive subpopulations of lung stem/progenitor cells (LSPCs) that are essential for lung epithelial maintenance and repair in vivo. Hence, LSPCs are in the center of interest of lung biology due to their promising therapeutic applications. To reach this goal, proper characterization of LSPCs, understanding of their proliferation and differentiation potentials and elucidation of mechanisms that control them are necessary. Therefore, development of reliable in vitro clonogenic assays has been needed. We established lungosphere assay, an in vitro sphere-forming 3D culture assay that enables to evaluate stem/progenitor cell activity, self-renewal and differentiation capacity of LSPCs and to conveniently test the effect of various treatments on LSPCs. Here we provide a detailed description of procedures for isolation of adult mouse lung epithelial cells, their culture in non-adherent conditions to form LSPC-derived spheroids (lungospheres) and for embedding of lungospheres into 3D extracellular matrix to model processes of lung tissue maintenance in a physiologically relevant microenvironment.

Czech name

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Czech description

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Type

D - Article in proceedings

CEP classification

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OECD FORD branch

10600 - Biological sciences

Project

Result was created during the realization of more than one project. More information in the Projects tab.

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year

2017

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Article name in the collection

3D Cell Culture : Methods and Protocols

ISBN

9781493970193

ISSN

1064-3745

e-ISSN

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Number of pages

17

Pages from-to

149-165

Publisher name

Humana Press

Place of publication

New York

Event location

New York

Event date

Jan 1, 2017

Type of event by nationality

CST - Celostátní akce

UT code for WoS article

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