Interaction of C-terminal p53 isoforms depends strongly upon DNA sequence and topology

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68081707%3A_____%2F23%3A00574514" target="_blank" >RIV/68081707:_____/23:00574514 - isvavai.cz</a>
Alternative codes found
RIV/62156489:43110/23:43922658 RIV/00216305:26210/22:PU146617
Result on the web
<a href="https://www.sciencedirect.com/science/article/pii/S0300908422003352?via%3Dihub" target="_blank" >https://www.sciencedirect.com/science/article/pii/S0300908422003352?via%3Dihub</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1016/j.biochi.2022.12.011" target="_blank" >10.1016/j.biochi.2022.12.011</a>

Result language
angličtina
Original language name
Interaction of C-terminal p53 isoforms depends strongly upon DNA sequence and topology
Original language description
The p53 protein is a key tumor suppressor and the most commonly mutated and down-regulated protein in human tumors. It functions mainly through interaction with DNA, and p53 acts as a transcription factor that recognizes the so-called p53 target sites on the promoters of various genes. P53 has been shown to exist as many isoforms, including three C-terminal isoforms that are produced by alternative splicing. Because the C-terminal domain is responsible for sequence-nonspecific binding and regulation of p53 binding, we have analyzed DNA recognition by these C-terminal isoforms. Using atomic force microscopy, we show for the first time that all C-terminal isoforms recognize superhelical DNA. It is particularly noteworthy that a sequence-specific p53 consensus binding site is bound by p53a and b isoforms with similar affinities, whilst p53a shows higher binding to a quadruplex sequence than both p53b and p53g, and p53g loses preferential binding to both the consensus binding sequence and the quadruplex-forming sequence. These results show the important role of the variable p53 C-terminal amino acid sequences for DNA recognition. (c) 2022 Elsevier B.V. and Societe Francaise de Biochimie et Biologie Moleculaire (SFBBM). All rights reserved.
Czech name
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Czech description
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Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
—
OECD FORD branch
10608 - Biochemistry and molecular biology

Project
<a href="/en/project/GA22-21903S" target="_blank" >GA22-21903S: Local DNA structures and their role in mutant p53 protein function in human tumours</a><br>
Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year
2023
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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