Genosensing on a 3D-printed nanocarbon electrode

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68081707%3A_____%2F23%3A00575169" target="_blank" >RIV/68081707:_____/23:00575169 - isvavai.cz</a>
Alternative codes found
RIV/61989100:27240/23:10253025 RIV/00216305:26620/23:PU150072
Result on the web
<a href="https://www.sciencedirect.com/science/article/pii/S1388248123000826?via%3Dihub" target="_blank" >https://www.sciencedirect.com/science/article/pii/S1388248123000826?via%3Dihub</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1016/j.elecom.2023.107508" target="_blank" >10.1016/j.elecom.2023.107508</a>

Result language
angličtina
Original language name
Genosensing on a 3D-printed nanocarbon electrode
Original language description
In this paper we present the characterization of 3D-printed nanocarbon electrodes (3DnCes) and their application in electrochemical enzyme-linked detection of DNA hybridization. The approach takes advantage of a facile procedure based on adsorption of target DNA on the electrode surface followed by hybridization with a biotinylated probe and binding of streptavidin-alkaline phosphatase conjugate. The alkaline phosphatase converts 1-naphthyl phosphate in the background electrolyte into electrochemically oxidizable 1-naphthol, which is subsequently detected using linear sweep voltammetry. The preparation, characterization, and analytical performance of the 3DnCes are reported. The results show the applicability of such 3DnCes in detection of target DNA hybridization specifically with the complementary biotinylated probe, and indicate the potential of 3D printed electrodes for use in various bioanalytical approaches.
Czech name
—
Czech description
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Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
—
OECD FORD branch
10405 - Electrochemistry (dry cells, batteries, fuel cells, corrosion metals, electrolysis)

Project
<a href="/en/project/GA20-03187S" target="_blank" >GA20-03187S: Study of electrochemical and adsorption processes on nanostructured sp2 and sp3 carbon surfaces in relation to their physico-chemical characteristics</a><br>
Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year
2023
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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