Intestinal Paneth cell differentiation relies on asymmetric regulation of Wnt signaling by Daam1/2
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68081707%3A_____%2F23%3A00583497" target="_blank" >RIV/68081707:_____/23:00583497 - isvavai.cz</a>
Alternative codes found
RIV/00216224:14310/23:00132483
Result on the web
<a href="https://www.science.org/doi/10.1126/sciadv.adh9673" target="_blank" >https://www.science.org/doi/10.1126/sciadv.adh9673</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1126/sciadv.adh9673" target="_blank" >10.1126/sciadv.adh9673</a>
Alternative languages
Result language
angličtina
Original language name
Intestinal Paneth cell differentiation relies on asymmetric regulation of Wnt signaling by Daam1/2
Original language description
The mammalian intestine is one of the most rapidly self-renewing tissues, driven by stem cells residing at the crypt bottom. Paneth cells form a major element of the niche microenvironment providing various growth factors to orchestrate intestinal stem cell homeostasis, such as Wnt3. Different Wnt ligands can selectively activate beta-catenin-dependent (canonical) orindependent (noncanonical) signaling. Here, we report that the Dishevelled-associated activator of morphogenesis 1 (Daam1) and its paralogue Daam2 asymmetrically regulate canonical and noncanonical Wnt (Wnt/PCP) signaling. Daam1/2 interacts with the Wnt inhibitor RNF43, and Daam1/2 double knockout stimulates canonical Wnt signaling by preventing RNF43-dependent degradation of the Wnt receptor, Frizzled (Fzd). Single-cell RNA sequencing analysis revealed that Paneth cell differentiation is impaired by Daam1/2 depletion because of defective Wnt/PCP signaling. Together, we identified Daam1/2 as an unexpected hub molecule coordinating both canonical and noncanonical Wnt, which is fundamental for specifying an adequate number of Paneth cells.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10609 - Biochemical research methods
Result continuities
Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2023
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Science Advances
ISSN
2375-2548
e-ISSN
2375-2548
Volume of the periodical
9
Issue of the periodical within the volume
47
Country of publishing house
US - UNITED STATES
Number of pages
18
Pages from-to
eadh9673
UT code for WoS article
001139850400006
EID of the result in the Scopus database
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