Factors influencing the efficacy of recombinant tissue plasminogen activator: Implications for ischemic stroke treatment

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68081707%3A_____%2F24%3A00587347" target="_blank" >RIV/68081707:_____/24:00587347 - isvavai.cz</a>

Alternative codes found

RIV/00216224:14110/24:00136220 RIV/00159816:_____/24:00081428

Result on the web

<a href="https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0302269" target="_blank" >https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0302269</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1371/journal.pone.0302269" target="_blank" >10.1371/journal.pone.0302269</a>

Result language

angličtina

Original language name

Factors influencing the efficacy of recombinant tissue plasminogen activator: Implications for ischemic stroke treatment

Original language description

Intravenous thrombolysis with a recombinant tissue plasminogen activator (rt-PA) is the first-line treatment of acute ischemic stroke. However, successful recanalization is relatively low and the underlying processes are not completely understood. The goal was to provide insights into clinically important factors potentially limiting rt-PA efficacy such as clot size, rt-PA concentration, clot age and also rt-PA in combination with heparin anticoagulant. We established a static in vitro thrombolytic model based on red blood cell (RBC) dominant clots prepared using spontaneous clotting from the blood of healthy donors. Thrombolysis was determined by clot mass loss and by RBC release. The rt-PA became increasingly less efficient for clots larger than 50 mu l at a clinically relevant concentration of 1.3 mg/l. A tenfold decrease or increase in concentration induced only a 2-fold decrease or increase in clot degradation. Clot age did not affect rt-PA-induced thrombolysis but 2-hours-old clots were degraded more readily due to higher activity of spontaneous thrombolysis, as compared to 5-hours-old clots. Finally, heparin (50 and 100 IU/ml) did not influence the rt-PA-induced thrombolysis. Our study provided in vitro evidence for a clot size threshold: clots larger than 50 mu l are hard to degrade by rt-PA. Increasing rt-PA concentration provided limited thrombolytic efficacy improvement, whereas heparin addition had no effect. However, the higher susceptibility of younger clots to thrombolysis may prompt a shortened time from the onset of stroke to rt-PA treatment.

Czech name

—

Czech description

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Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

—

OECD FORD branch

30205 - Hematology

Project

Result was created during the realization of more than one project. More information in the Projects tab.

Continuities

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2024

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

PLoS ONE

ISSN

1932-6203

e-ISSN

1932-6203

Volume of the periodical

19

Issue of the periodical within the volume

6

Country of publishing house

US - UNITED STATES

Number of pages

13

Pages from-to

e0302269

UT code for WoS article

001241954400011

EID of the result in the Scopus database

2-s2.0-85195438931