Non-canonical bases differentially represented in the sex chromosomes of the dioecious plant Silene latifolia
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68081707%3A_____%2F24%3A00599220" target="_blank" >RIV/68081707:_____/24:00599220 - isvavai.cz</a>
Alternative codes found
RIV/00216224:14310/24:00135887
Result on the web
<a href="https://academic.oup.com/jxb/article/75/13/3849/7656699" target="_blank" >https://academic.oup.com/jxb/article/75/13/3849/7656699</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1093/jxb/erae178" target="_blank" >10.1093/jxb/erae178</a>
Alternative languages
Result language
angličtina
Original language name
Non-canonical bases differentially represented in the sex chromosomes of the dioecious plant Silene latifolia
Original language description
The oxidation of 5-methylcytosine (5mC) to 5-hydroxymethylcytosine (5hmC), 5-formylcytosine (5fC), and 5-carboxylcytosine (5caC), known as oxi-mCs, garners significant interest in plants as potential epigenetic marks. While research in mammals has established a role in cell reprogramming, carcinogenesis, and gene regulation, their functions in plants remain unclear. In rice, 5hmC has been associated with transposable elements (TEs) and heterochromatin. This study utilizes Silene latifolia, a dioecious plant with heteromorphic sex chromosomes and a genome with a large proportion of TEs, which provides a favourable environment for the study of oxi-mCs in individual sexes. Notably, we detected surprisingly high levels of oxi-mCs in S. latifolia comparable with mammals. Nuclei showed enrichment in heterochromatic regions, except for 5hmC whose signal was homogeneously distributed. Intriguingly, the same X chromosome in females displayed overall enrichment of 5hmC and 5fC compared with its counterpart. This fact is shared with 5mC, resembling dosage compensation. Co-localization showed higher correlation between 5mC and 5fC than with 5hmC, indicating no potential relationship between 5hmC and 5fC. Additionally, the promoter of several sex-linked genes and sex-biased TEs clustered in a clear sex-dependent way. Together, these findings unveil a hypothetical role for oxi-mCs in S. latifolia sex chromosome development, warranting further exploration.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10611 - Plant sciences, botany
Result continuities
Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2024
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Journal of Experimental Botany
ISSN
0022-0957
e-ISSN
1460-2431
Volume of the periodical
75
Issue of the periodical within the volume
13
Country of publishing house
US - UNITED STATES
Number of pages
13
Pages from-to
3849-3861
UT code for WoS article
001243510300001
EID of the result in the Scopus database
2-s2.0-85198616427