In Planta Reporter Assay Demonstrated the Inherent Potential of Turmeric as a Natural Hypo-Methylating Agent in Cultivated Tobacco (Nicotiana tabacum L.)
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68081707%3A_____%2F24%3A00618134" target="_blank" >RIV/68081707:_____/24:00618134 - isvavai.cz</a>
Result on the web
<a href="https://link.springer.com/article/10.1134/S1021443724608279" target="_blank" >https://link.springer.com/article/10.1134/S1021443724608279</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1134/S1021443724608279" target="_blank" >10.1134/S1021443724608279</a>
Alternative languages
Result language
angličtina
Original language name
In Planta Reporter Assay Demonstrated the Inherent Potential of Turmeric as a Natural Hypo-Methylating Agent in Cultivated Tobacco (Nicotiana tabacum L.)
Original language description
The current research seeks to assess the inherent potential (if any) of turmeric (TMC) as an epigenetic modulator in plants. To monitor its specific effects on gene-activation, we used a well-established in planta reporter assay system based on cultivated tobacco (Nicotiana tabacum L.) as a model system harbouring an epigenetically-silenced (hyper-methylation in specific) neomycin phosphotransferase (nptII) transgene driven by cauliflower mosaic virus 35S promoter (P-CaMV35S). Our results showed that the exposure of tobacco plantlets to an empirically optimized concentration [i.e., 2.5% (v/v)] of aqueous TMC extract (TMCE) causes the: (1) restoration of non-silenced plant phenotype on kanamycin selection media, (2) induced steady-state nptII transcript levels and (3) DNA hypo-methylation of 5'-UTR nptII sub-region. Based on these observations, a general role of TMC as a DNA hypo-methylating agent in plants growth and development has been anticipated.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10611 - Plant sciences, botany
Result continuities
Project
<a href="/en/project/GA20-28029S" target="_blank" >GA20-28029S: Relevance of duplicated promoter sequences for the function of plant 35S rDNA intergenic spacers.</a><br>
Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2024
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Russian Journal of Plant Physiology
ISSN
1021-4437
e-ISSN
1608-3407
Volume of the periodical
71
Issue of the periodical within the volume
6
Country of publishing house
RU - RUSSIAN FEDERATION
Number of pages
7
Pages from-to
198
UT code for WoS article
001412539000030
EID of the result in the Scopus database
2-s2.0-85218160266