The<i> Drosophila</i> RNA binding protein Hrp48 binds a specific RNA sequence of the<i> msl-2</i> mRNA 3' UTR to regulate translation
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68081707%3A_____%2F25%3A00601630" target="_blank" >RIV/68081707:_____/25:00601630 - isvavai.cz</a>
Result on the web
<a href="https://www.sciencedirect.com/science/article/pii/S0301462224001753?via%3Dihub" target="_blank" >https://www.sciencedirect.com/science/article/pii/S0301462224001753?via%3Dihub</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1016/j.bpc.2024.107346" target="_blank" >10.1016/j.bpc.2024.107346</a>
Alternative languages
Result language
angličtina
Original language name
The<i> Drosophila</i> RNA binding protein Hrp48 binds a specific RNA sequence of the<i> msl-2</i> mRNA 3' UTR to regulate translation
Original language description
Repression of msl-2 mRNA translation is essential for viability of Drosophila melanogaster females to prevent hypertranscription of both X chromosomes. This translational control event is coordinated by the female-specific protein Sex-lethal (Sxl) which recruits the RNA binding proteins Unr and Hrp48 to the 3' untranslated region (UTR) of the msl-2 transcript and represses translation initiation. The mechanism exerted by Hrp48 during translation repression and its interaction with msl-2 are not well understood. Here we investigate the RNA binding specificity and affinity of the tandem RNA recognition motifs of Hrp48. Using NMR spectroscopy, molecular dynamics simulations and isothermal titration calorimetry, we identified the exact region of msl-2 3' UTR recognized by Hrp48. Additional biophysical experiments and translation assays give further insights into complex formation of Hrp48, Unr, Sxl and RNA. Our results show that Hrp48 binds independent of Sxl and Unr downstream of the E and F binding sites of Sxl and Unr to msl-2.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10608 - Biochemistry and molecular biology
Result continuities
Project
<a href="/en/project/GA23-05639S" target="_blank" >GA23-05639S: Molecular dynamics simulations of RNA: from static structures to molecular ensembles</a><br>
Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2025
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Biophysical Chemistry
ISSN
0301-4622
e-ISSN
1873-4200
Volume of the periodical
316
Issue of the periodical within the volume
JAN 2025
Country of publishing house
NL - THE KINGDOM OF THE NETHERLANDS
Number of pages
15
Pages from-to
107346
UT code for WoS article
001354372800001
EID of the result in the Scopus database
2-s2.0-85208096293