Dynamic modification of proteins for fluorometric detection in CZE.
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68081715%3A_____%2F01%3A25010050" target="_blank" >RIV/68081715:_____/01:25010050 - isvavai.cz</a>
Result on the web
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DOI - Digital Object Identifier
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Alternative languages
Result language
angličtina
Original language name
Dynamic modification of proteins for fluorometric detection in CZE.
Original language description
The separation techniques employing fluorescence detection are sensitive and selective so they have been often applied for the trace analysis of biological samples. The commonly used derivatization of proteins can lower the detection limits; however, they can change the acido-basic properties and mobilities when compared to the native species. Recently, we have used the colored tenside as a buffer additive for the photometric detection of proteins in the UV region. The selectivity, efficiency and resolution of CZE separation using this dye were found to be similar to the CZE with SDS as the additive. In this study, the ampiphilic fluorescent compound is suggested as a buffer additive in CZE for dynamic modification of the sample of several proteins. Using the deuterium lamp for the excitation in the UV region for the on column fluorometric detection, the amol minimum detectable amounts of the proteins sampled on the CZE capillary were achieved.
Czech name
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Czech description
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Classification
Type
D - Article in proceedings
CEP classification
CB - Analytical chemistry, separation
OECD FORD branch
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Result continuities
Project
<a href="/en/project/IAA4031901" target="_blank" >IAA4031901: Capillary isotachoforesis with combined continuous conductivity and pH gradients</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Others
Publication year
2001
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Article name in the collection
2nd International Symposium Separations in the BioSciences. SBS 2001.
ISBN
80-7080-437-8
ISSN
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e-ISSN
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Number of pages
1
Pages from-to
91
Publisher name
N/A
Place of publication
Praha
Event location
Praha [CZ]
Event date
Sep 17, 2001
Type of event by nationality
EUR - Evropská akce
UT code for WoS article
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