Decreased protein peak asymmetry and width due to static capillary coating with hydrophilic derivatives of polydimethylacrylamide.

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68081715%3A_____%2F02%3A25020018" target="_blank" >RIV/68081715:_____/02:25020018 - isvavai.cz</a>

Result on the web

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DOI - Digital Object Identifier

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Result language

angličtina

Original language name

Decreased protein peak asymmetry and width due to static capillary coating with hydrophilic derivatives of polydimethylacrylamide.

Original language description

Protein CZE peaks in the EPDMA-coated capillaries exhibited a peak asymmetry similar to that obtained in capillaries with 'covalent static' coating of polyacrylamide, suggesting a similar degree of adsorption of the protein onto the coating. Instabilityof such coating at very low ionic strength and its stripping from the capillary in the presence of SDS also indicated a hydrophilic bonding of EPDMA to the silanol surface of the capillary, while its stripping in the CZE of 'carboxylate-modified' polystyrene suggested a competition between carboxylate and EPDMA for the hydrophilic bonds to silanol. To test those propositions, a number of EPDMA-derived coating agents with increased hydrophilicity were syntesized. Of a number of the hydrophilic coating agents tested, only two exhibited for representative proteins a decreased peak asymmetry and width while the stability of the suppresion of EOF, and the stability of mobility in consecutive CZE runs was reduced relative to EPDMA. Coating ag

Czech name

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Czech description

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Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

CB - Analytical chemistry, separation

OECD FORD branch

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Project

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Continuities

Z - Vyzkumny zamer (s odkazem do CEZ)

Publication year

2002

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Electrophoresis

ISSN

0173-0835

e-ISSN

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Volume of the periodical

23

Issue of the periodical within the volume

14

Country of publishing house

DE - GERMANY

Number of pages

5

Pages from-to

2274-2278

UT code for WoS article

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EID of the result in the Scopus database

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