Sol-gel column technology for capillary isoelectric focusing of microorganisms and biopolymers with UV or fluorometric detection.

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68081715%3A_____%2F03%3A25033013" target="_blank" >RIV/68081715:_____/03:25033013 - isvavai.cz</a>

Alternative codes found

RIV/00216224:14110/03:00008624 RIV/00216224:14110/03:00019260

Result on the web

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DOI - Digital Object Identifier

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Result language

angličtina

Original language name

Sol-gel column technology for capillary isoelectric focusing of microorganisms and biopolymers with UV or fluorometric detection.

Original language description

The sol-gel surface modification is used for capillary isoelectric focusing of microorganisms and biopolymers. The coating strongly decreases the electroosmotic flow so that it enables the use of the short capillaries down to 100 mm in the separation length. The examples of capillary isoelectric focusing of the low-molecular-mass pI markers and mixed cultures of microbial populations of Escherichia coli, Candida albicans, Staphylococcus epidermidis and Enteroccocus faecalis with UV detection are shown.It is possible to quantify bacterial cells according their peak areas; the minimum detectable number of microbial cells was 5 x 102 1 x 103. The compatibility of sol-gel capillaries with sensitive fluorometric detection of fluorescent pI markers togetherwith fluorescein labeled proteins is demonstrated.

Czech name

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Czech description

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Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

CB - Analytical chemistry, separation

OECD FORD branch

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Project

Result was created during the realization of more than one project. More information in the Projects tab.

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>Z - Vyzkumny zamer (s odkazem do CEZ)

Publication year

2003

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Electrophoresis

ISSN

0173-0835

e-ISSN

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Volume of the periodical

24

Issue of the periodical within the volume

9

Country of publishing house

DE - GERMANY

Number of pages

8

Pages from-to

1383-1390

UT code for WoS article

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EID of the result in the Scopus database

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