Optimized method for identification of the proteomes secreted by cardiac cells

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68081715%3A_____%2F13%3A00392231" target="_blank" >RIV/68081715:_____/13:00392231 - isvavai.cz</a>

Result on the web

<a href="http://dx.doi.org/10.1007/978-1-62703-386-2_18" target="_blank" >http://dx.doi.org/10.1007/978-1-62703-386-2_18</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1007/978-1-62703-386-2_18" target="_blank" >10.1007/978-1-62703-386-2_18</a>

Result language

angličtina

Original language name

Optimized method for identification of the proteomes secreted by cardiac cells

Original language description

There are several crucial steps that have to be followed to ensure successful secreted proteome detection and identification. Simultaneously with the optimization of the experimental conditions for various cell type culturing and subsequent cell conditioning to obtain conditioned medium with secreted proteins in vitro, the analytical separation methods for fractionation of complex protein mixture and mass spectrometry for protein identification are of high importance. Although optimized for neonatal cardiac myocytes, the general steps described in the following chapter can be adapted to other cell types as well.

Czech name

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Czech description

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Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

CB - Analytical chemistry, separation

OECD FORD branch

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Project

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Continuities

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2013

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Methods in molecular biology

ISSN

1940-6029

e-ISSN

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Volume of the periodical

1005

Issue of the periodical within the volume

1005

Country of publishing house

US - UNITED STATES

Number of pages

11

Pages from-to

225-235

UT code for WoS article

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EID of the result in the Scopus database

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