Simple route of caspase-3 FRET sensor synthesis using ?click chemistry
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68081715%3A_____%2F15%3A00448949" target="_blank" >RIV/68081715:_____/15:00448949 - isvavai.cz</a>
Result on the web
<a href="http://www.ce-ce.org/CECE2015/CECE%202015%20proceedings_full.pdf" target="_blank" >http://www.ce-ce.org/CECE2015/CECE%202015%20proceedings_full.pdf</a>
DOI - Digital Object Identifier
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Alternative languages
Result language
angličtina
Original language name
Simple route of caspase-3 FRET sensor synthesis using ?click chemistry
Original language description
Programmed cell death or apoptosis is regulated process of cell suicide. The central role in apoptosis play cysteine proteases called caspases. Caspases recognize tetra-peptide sequences Asp-Glu-Val-Asp (DEVD) on their substrates and hydrolyze peptide bonds after aspartic acid residues. Various techniques for the determination of caspase-3 are commercially available e.g. Enzyme Linked Immuno-Sorbent Assay (ELISA), Western blotting or flow cytometric analysis. The products of the cleavage can be detectedby spectrophotometry, fluorimetry, chemiluminescence (CL) or ELISA. In this work, we suggested fluorescent sensor based on easily prepared Förster Resonance Energy Transfer (FRET). We use very simple chemistry called ?click. This type of chemistry takesadvantages of quickness, simplicity and cheapness. ?Click chemistry is based on usage of various functional group and cross-linkers to combine individual molecules together.
Czech name
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Czech description
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Classification
Type
D - Article in proceedings
CEP classification
CB - Analytical chemistry, separation
OECD FORD branch
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Result continuities
Project
<a href="/en/project/GA14-28254S" target="_blank" >GA14-28254S: Single cell analysis of signaling molecules</a><br>
Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2015
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Article name in the collection
CECE 2015. 12th International Interdisciplinary Meeting on Bioanalysis
ISBN
978-80-904959-3-7
ISSN
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e-ISSN
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Number of pages
4
Pages from-to
137-140
Publisher name
Ústav analytické chemie AV ČR, v. v. i
Place of publication
Brno
Event location
Brno
Event date
Sep 21, 2015
Type of event by nationality
WRD - Celosvětová akce
UT code for WoS article
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