Cereal n-glycoproteins enrichment by lectin affinity monolithic chromatography
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68081715%3A_____%2F16%3A00460139" target="_blank" >RIV/68081715:_____/16:00460139 - isvavai.cz</a>
Result on the web
<a href="http://dx.doi.org/10.1556/0806.44.2016.019" target="_blank" >http://dx.doi.org/10.1556/0806.44.2016.019</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1556/0806.44.2016.019" target="_blank" >10.1556/0806.44.2016.019</a>
Alternative languages
Result language
angličtina
Original language name
Cereal n-glycoproteins enrichment by lectin affinity monolithic chromatography
Original language description
Research of cereal glycoproteins is important for understanding of their functional properties, their role during technological processing of cereals and it can serve as a valuable tool for the detection of cereal allergens. The main intention of this study was the screening of profile of water-soluble glycoproteins present in barley (Hordeum vulgare), wheat durum (Triticum durum) and spelt (Triticum spelta). Lectin monolithic HPLC column was used for rapid and effective enrichment of glycoprotein fractions. Captured glycoproteins were electrophoretically separated and analyzed by MALDI-TOF MS. Presented procedure resulted in identification of a group of N-glycoprotein candidates with affinity to lectin concanavalin A (ConA). Such molecules could have, among others, an allergenic potential.
Czech name
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Czech description
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Classification
Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
CB - Analytical chemistry, separation
OECD FORD branch
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Result continuities
Project
<a href="/en/project/GPP503%2F12%2FP395" target="_blank" >GPP503/12/P395: Searching for glycosylated allergens in plants important for food chemistry via modern proteomic approaches</a><br>
Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2016
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Cereal Research Communications
ISSN
0133-3720
e-ISSN
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Volume of the periodical
44
Issue of the periodical within the volume
2
Country of publishing house
HU - HUNGARY
Number of pages
12
Pages from-to
286-297
UT code for WoS article
000388000600010
EID of the result in the Scopus database
2-s2.0-84971406539