Capillary isoelectric focusing-useful tool for detection and quantification of lactic acid bacteria in milk

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68081715%3A_____%2F16%3A00464040" target="_blank" >RIV/68081715:_____/16:00464040 - isvavai.cz</a>
Alternative codes found
RIV/00159816:_____/16:00066209 RIV/00216224:14110/16:00088929 RIV/26722861:_____/16:N0000026
Result on the web
<a href="http://dx.doi.org/10.1007/s12161-016-0522-6" target="_blank" >http://dx.doi.org/10.1007/s12161-016-0522-6</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1007/s12161-016-0522-6" target="_blank" >10.1007/s12161-016-0522-6</a>

Result language
angličtina
Original language name
Capillary isoelectric focusing-useful tool for detection and quantification of lactic acid bacteria in milk
Original language description
The lactic acid bacteria (LAB), including lactobacilli and enterococci, represent an important part of normal microflora in humans. Simultaneously, they are frequently used as probiotics and in food industry for production of fermented milk products. The rapid and sensitive detection of these microorganisms is crucial for the quality control. In this study, the capillary isoelectric focusing (CIEF) was successfully used for the separation and characterization of probiotic LAB, L. paracasei sp. paracasei; L. acidifarinae; L. fermentum; L. gasseri; L. helveticus; L. plantarum; L. delbrueckii; L. salivarius; E. durans; E. faecalis; and E. faecium, according to their isoelectric points (pI). All pIs of lactobacilli and enterococci were found in the acidic part of the pH range. Subsequently, the milk samples spiked with L. rhamnosus, and E. faecium were analyzed by CIEF. The optimal adjustment of the separation conditions allowed detection and quantification of the bacteria in the sample with sufficient sensitivity. Therefore, CIEF is an efficient approach to rapid detection and separation of LAB even directly in milk products in future.
Czech name
—
Czech description
—

Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
CB - Analytical chemistry, separation
OECD FORD branch
—

Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year
2016
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Similar results(10)