Design and validation of an STR hexaplex assay for DNA profiling of grapevine cultivars
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68081715%3A_____%2F16%3A00465488" target="_blank" >RIV/68081715:_____/16:00465488 - isvavai.cz</a>
Alternative codes found
RIV/62156489:43510/16:43910458 RIV/61989592:15110/16:33161188 RIV/61989592:15310/16:33161188
Result on the web
<a href="http://dx.doi.org/10.1002/elps.201600068" target="_blank" >http://dx.doi.org/10.1002/elps.201600068</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1002/elps.201600068" target="_blank" >10.1002/elps.201600068</a>
Alternative languages
Result language
angličtina
Original language name
Design and validation of an STR hexaplex assay for DNA profiling of grapevine cultivars
Original language description
Although the analysis of length polymorphism at STR loci has become amethod of choice for grape cultivar identification, the standardization of methods for this purpose lags behind that of methods for DNA profiling in human and animal forensic genetics. The aim of this study was thus to design and validate a grapevine STR protocol with a practically useful level of multiplexing. Using free bioinformatics tools, published primer sequences, and nucleotide databases, we constructed and optimized a primer set for the simultaneous analysis of six STR loci (VVIi51, scu08vv, scu05vv, VVMD17, VrZAG47, and VrZAG83) by multiplex PCR and CE with laser-induced fluorescence, and tested it on 90 grape cultivars. The new protocol requires subnanogram quantities of the DNA template and enables automated, high-throughput genetic analysis with reasonable discriminatory power. As such, it represents a step toward further standardization of grape DNA profiling.
Czech name
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Czech description
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Classification
Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
CB - Analytical chemistry, separation
OECD FORD branch
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Result continuities
Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2016
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Electrophoresis
ISSN
0173-0835
e-ISSN
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Volume of the periodical
37
Issue of the periodical within the volume
23-24
Country of publishing house
DE - GERMANY
Number of pages
9
Pages from-to
3059-3067
UT code for WoS article
000392421400003
EID of the result in the Scopus database
2-s2.0-84995791191