Identification of bacterial uropathogens by preparative isoelectricfocusing and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68081715%3A_____%2F18%3A00484140" target="_blank" >RIV/68081715:_____/18:00484140 - isvavai.cz</a>
Alternative codes found
RIV/00216224:14110/18:00106895 RIV/00159816:_____/18:00068884
Result on the web
<a href="http://dx.doi.org/10.1016/j.chroma.2017.11.072" target="_blank" >http://dx.doi.org/10.1016/j.chroma.2017.11.072</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1016/j.chroma.2017.11.072" target="_blank" >10.1016/j.chroma.2017.11.072</a>

Result language
angličtina
Original language name
Identification of bacterial uropathogens by preparative isoelectricfocusing and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry
Original language description
This study describes a new method for simultaneous identification of uropathogens in the case of poly-bacterial urinary tract infections. The method utilizes recently developed preparative isoelectric focusing(IEF) in cellulose-based separation medium with a subsequent analysis by matrix-assisted laser desorp-tion/ionization time-of-flight mass spectrometry (MALDI-TOF MS). Preparative IEF was successfully usedfor both purification and separation of bacteria, Escherichia coli (pI 4.6) and Staphylococcus aureus (pI 3.4),in urine samples. The focused zones of bacteria, localized by the positions of focused colored pI markers,were easily collected from the separation media after the IEF analysis and then unambiguously identifiedby MALDI-TOF MS. The proposed method enables the identification of bacteria in urine specimens whenthe concentration of individual bacteria is ≥104cells mL−1. Another benefit is the viability of bacteriaextracted from the collected fractions after preparative IEF.
Czech name
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Czech description
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Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10406 - Analytical chemistry

Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year
2018
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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