Identification of Lactobacillus spp. isolates from children's intestinal tissues by MALDI-TOF MS profiling and automated ribotyping

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14310%2F11%3A00054001" target="_blank" >RIV/00216224:14310/11:00054001 - isvavai.cz</a>
Result on the web
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DOI - Digital Object Identifier
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Result language
angličtina
Original language name
Identification of Lactobacillus spp. isolates from children's intestinal tissues by MALDI-TOF MS profiling and automated ribotyping
Original language description
Background: In clinical microbiology as well as in dairy industry there is a great need for reliable identification and characterization of lactic acid bacteria. Matrix Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry (MALDI TOF MS)represents a fast, procedurally simple and sensitive chemotaxonomical method with a high discriminatory power. The identification of bacteria is based on characteristic and reproducible peptide/protein fingerprints. Objectives: The aim of this work was to assess MALDI TOF MS profiling and automatic ribotyping as tools for reliable identification of Lactobacillus isolates from human intestinal tissues. Methods: MALDI-TOF mass spectra of 57 Lactobacillus strains were obtained with an Ultraflex III instrument (Bruker Daltonik). BioTyper software (Bruker Daltonik) involving a database of lactic acid bacteria MALDI-TOF MS profiles was used for data processing.
Czech name
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Czech description
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Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>Z - Vyzkumny zamer (s odkazem do CEZ)

Publication year
2011
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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