Preparation and Analysis of Quantum Dots: Applications of Capillary Electrophoresis
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68081715%3A_____%2F20%3A00544799" target="_blank" >RIV/68081715:_____/20:00544799 - isvavai.cz</a>
Alternative codes found
RIV/00216305:26620/20:PU136515
Result on the web
<a href="https://link.springer.com/protocol/10.1007%2F978-1-0716-0463-2_3#citeas" target="_blank" >https://link.springer.com/protocol/10.1007%2F978-1-0716-0463-2_3#citeas</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1007/978-1-0716-0463-2_3" target="_blank" >10.1007/978-1-0716-0463-2_3</a>
Alternative languages
Result language
angličtina
Original language name
Preparation and Analysis of Quantum Dots: Applications of Capillary Electrophoresis
Original language description
The implementation of quantum dots in analytical chemistry has already advanced from basic research activities to routine applications of commercially available fluorescent agents present in sophisticated assays kits. Nevertheless, a further development of new preparation and characterization methods of nanoparticles is still required to increase the sensitivity of analytical methods substantially. Thus, in many bioanalytical applications, important molecules such as DNA, proteins, and antibodies are routinely conjugated with fluorescent tags to reach even the absolute sensitivity, that is, the capability to detect a single molecule in complex matrices. Semiconductor quantum dots have already proved to be suitable components of highly luminescent tags, probes, and sensors with broad applicability in analytical chemistry. Quantum dots provide high extinction coefficients together with wide ranges of excitation wavelengths, size- and composition-tunable emissions, narrow and symmetric emission spectra, good quantum yields, relatively long size-dependent luminescence lifetime, and low photobleaching. Most of these properties are superior when compared with conventional organic fluorescent dyes. In this chapter, optimized procedures for the preparation of water-dispersed CdTe quantum dots, their coatings and conjugation reactions with antibodies, DNA, and macrocycles, and their analyses by capillary electrophoresis are described. The potential of capillary electrophoresis for fast analyses of nanoparticles, their conjugates with antibodies and immunocomplexes with targeted antigens, is demonstrated as an example.
Czech name
—
Czech description
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Classification
Type
C - Chapter in a specialist book
CEP classification
—
OECD FORD branch
10406 - Analytical chemistry
Result continuities
Project
—
Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2020
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Book/collection name
Quantum Dots
ISBN
978-1-0716-0462-5
Number of pages of the result
29
Pages from-to
"Roč. 2135 (2020)"
Number of pages of the book
308
Publisher name
Humana Press
Place of publication
New York
UT code for WoS chapter
000680965100004