An advanced conjugation strategy for the preparation of quantum dot-antibody immunoprobes
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216275%3A25310%2F17%3A39911198" target="_blank" >RIV/00216275:25310/17:39911198 - isvavai.cz</a>
Alternative codes found
RIV/68081715:_____/17:00473595
Result on the web
<a href="http://dx.doi.org/10.1039/c6ay03322a" target="_blank" >http://dx.doi.org/10.1039/c6ay03322a</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1039/c6ay03322a" target="_blank" >10.1039/c6ay03322a</a>
Alternative languages
Result language
angličtina
Original language name
An advanced conjugation strategy for the preparation of quantum dot-antibody immunoprobes
Original language description
An advanced site-specific conjugation strategy for the preparation of quantum dot-based antibody probes applicable in various immunoassays from fluorescence to electrochemical biosensors is described. The combination of antigen (protein ApoE) modified magnetic particles providing protection to antibody binding sites, simple carbodiimide chemistry and carboxylate quantum dots (QDs) made of CdSe/ZnS is used for efficient labelling of anti-ApoE antibodies representing a model system. Polyacrylamide gel electrophoresis, fluorescence spectra measurements, capillary electrophoresis-laser induced fluorescence (CE-LIF) and square wave anodic stripping voltammetry (SWASV) were used for experimental verification of labelling efficiency. A simple change in antibody type makes this approach versatile and exploitable in a wide range of applications.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10620 - Other biological topics
Result continuities
Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Others
Publication year
2017
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Analytical Methods
ISSN
1759-9660
e-ISSN
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Volume of the periodical
9
Issue of the periodical within the volume
13
Country of publishing house
GB - UNITED KINGDOM
Number of pages
7
Pages from-to
1991-1997
UT code for WoS article
000399912500003
EID of the result in the Scopus database
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