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An advanced conjugation strategy for the preparation of quantum dot-antibody immunoprobes

The result's identifiers

  • Result code in IS VaVaI

    <a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216275%3A25310%2F17%3A39911198" target="_blank" >RIV/00216275:25310/17:39911198 - isvavai.cz</a>

  • Alternative codes found

    RIV/68081715:_____/17:00473595

  • Result on the web

    <a href="http://dx.doi.org/10.1039/c6ay03322a" target="_blank" >http://dx.doi.org/10.1039/c6ay03322a</a>

  • DOI - Digital Object Identifier

    <a href="http://dx.doi.org/10.1039/c6ay03322a" target="_blank" >10.1039/c6ay03322a</a>

Alternative languages

  • Result language

    angličtina

  • Original language name

    An advanced conjugation strategy for the preparation of quantum dot-antibody immunoprobes

  • Original language description

    An advanced site-specific conjugation strategy for the preparation of quantum dot-based antibody probes applicable in various immunoassays from fluorescence to electrochemical biosensors is described. The combination of antigen (protein ApoE) modified magnetic particles providing protection to antibody binding sites, simple carbodiimide chemistry and carboxylate quantum dots (QDs) made of CdSe/ZnS is used for efficient labelling of anti-ApoE antibodies representing a model system. Polyacrylamide gel electrophoresis, fluorescence spectra measurements, capillary electrophoresis-laser induced fluorescence (CE-LIF) and square wave anodic stripping voltammetry (SWASV) were used for experimental verification of labelling efficiency. A simple change in antibody type makes this approach versatile and exploitable in a wide range of applications.

  • Czech name

  • Czech description

Classification

  • Type

    J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database

  • CEP classification

  • OECD FORD branch

    10620 - Other biological topics

Result continuities

  • Project

    Result was created during the realization of more than one project. More information in the Projects tab.

  • Continuities

    P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Others

  • Publication year

    2017

  • Confidentiality

    S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Data specific for result type

  • Name of the periodical

    Analytical Methods

  • ISSN

    1759-9660

  • e-ISSN

  • Volume of the periodical

    9

  • Issue of the periodical within the volume

    13

  • Country of publishing house

    GB - UNITED KINGDOM

  • Number of pages

    7

  • Pages from-to

    1991-1997

  • UT code for WoS article

    000399912500003

  • EID of the result in the Scopus database