Elegant, rapid and simple procedure for labeling of antibodies with fluorescent ZnCd/S quantum dots for laser ablation ICP-MS detection

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F62156489%3A43210%2F21%3A43919822" target="_blank" >RIV/62156489:43210/21:43919822 - isvavai.cz</a>
Alternative codes found
RIV/00216305:26620/21:PU141193 RIV/00216224:14310/21:00123819
Result on the web
<a href="https://doi.org/10.37904/nanocon.2020.3752" target="_blank" >https://doi.org/10.37904/nanocon.2020.3752</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.37904/nanocon.2020.3752" target="_blank" >10.37904/nanocon.2020.3752</a>

Result language
angličtina
Original language name
Elegant, rapid and simple procedure for labeling of antibodies with fluorescent ZnCd/S quantum dots for laser ablation ICP-MS detection
Original language description
Antibody labeling with a signal-providing tag (e.g. an enzyme, chromophore, fluorophore, metal chelate, radioisotope, etc.) is a key procedure in bioanalytical techniques such an enzyme-linked immunosorbent assay, western blot, or immunohistochemical analysis. The process may be laborious, impair the activity, and time-consuming. Concisely, in this work, an elegant method of rapid and effective antibody labeling by quantum dots was developed taking advantage of partial reduction of antibody structure and UV-induced quantum dot formation. Zinc and cadmium with the thiol group of the antibody as capping agents stabilizing the fluorescent nanoparticle while the biorecognition capabilities were maintained. The ZnCd/S quantum dots creation has to prosper detected with capillary electrophoresis (CE) and laser ablation ICP- MS (LA- ICP- MS).
Czech name
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Czech description
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Publication year
2021
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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