Elegant, rapid and simple procedure for labeling of antibodies with fluorescent ZnCd/S quantum dots for laser ablation ICP-MS detection

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216305%3A26620%2F21%3APU141193" target="_blank" >RIV/00216305:26620/21:PU141193 - isvavai.cz</a>

Alternative codes found

RIV/62156489:43210/21:43919822 RIV/00216224:14310/21:00123819

Result on the web

<a href="https://www.confer.cz/nanocon/2020/3752-elegant-rapid-and-simple-procedure-for-labeling-of-antibodies-with-fluorescent-cdzn-s-nanoparticles-for-la-icp-ms-detection" target="_blank" >https://www.confer.cz/nanocon/2020/3752-elegant-rapid-and-simple-procedure-for-labeling-of-antibodies-with-fluorescent-cdzn-s-nanoparticles-for-la-icp-ms-detection</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.37904/nanocon.2020.3752" target="_blank" >10.37904/nanocon.2020.3752</a>

Result language

angličtina

Original language name

Elegant, rapid and simple procedure for labeling of antibodies with fluorescent ZnCd/S quantum dots for laser ablation ICP-MS detection

Original language description

Antibody labeling with a signal-providing tag (e.g. an enzyme, chromophore, fluorophore, metal chelate, radioisotope, etc.) is a key procedure in bioanalytical techniques such an enzyme-linked immunosorbent assay, western blot, or immunohistochemical analysis. The process may be laborious, impair the activity, and time-consuming. Concisely, in this work, an elegant method of rapid and effective antibody labeling by quantum dots was developed taking advantage of partial reduction of antibody structure and UV-induced quantum dot formation. Zinc and cadmium with the thiol group of the antibody as capping agents stabilizing the fluorescent nanoparticle while the biorecognition capabilities were maintained. The ZnCd/S quantum dots creation has to prosper detected with capillary electrophoresis (CE) and laser ablation ICP- MS (LA- ICP- MS).

Czech name

—

Czech description

—

Type

D - Article in proceedings

CEP classification

—

OECD FORD branch

10406 - Analytical chemistry

Project

—

Continuities

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2021

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Article name in the collection

NANOCON 2020 CONFERENCE PROCEEDINGS

ISBN

978-80-87294-98-7

ISSN

2694-930X

e-ISSN

—

Number of pages

4

Pages from-to

395-398

Publisher name

Tanger

Place of publication

neuveden

Event location

Brno, Hotel Voroněž

Event date

Oct 21, 2020

Type of event by nationality

WRD - Celosvětová akce

UT code for WoS article

000664505500067