Epitachophoretic preconcentration of proteins

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68081715%3A_____%2F21%3A00546004" target="_blank" >RIV/68081715:_____/21:00546004 - isvavai.cz</a>
Alternative codes found
RIV/00216224:14310/21:00124427
Result on the web
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DOI - Digital Object Identifier
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Result language
angličtina
Original language name
Epitachophoretic preconcentration of proteins
Original language description
In this work, we present a developed method for preconcentration of standards of proteins cytochrome c, myoglobin, and hemoglobin. Firstly, the electrolyte system was selected and optimized to provide low analysis time, narrow shape of the protein zone, and eliminate overheating at the center of the device. In the next step, the developed method was tested on the preconcentration of proteins: cytochrome c, myoglobin, and hemoglobin. After several adjustments myoglobin and hemoglobin were also uccessfully preconcentrated. After evaluation of pilot results, this technique provides up to fiftyfold preconcentration and recovery of over 70 % for each protein. The next plan is to preconcentrate proteins from complex biological matrices such as urine or blood plasma.
Czech name
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Czech description
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Project
<a href="/en/project/EF16_026%2F0008446" target="_blank" >EF16_026/0008446: Signal integration and epigenetic reprograming for plant productivity</a><br>
Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year
2021
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Article name in the collection
Proceedings of the 17th International Students Conference “Modern Analytical Chemistry”
ISBN
978-80-7444-089-2
ISSN
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e-ISSN
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Number of pages
6
Pages from-to
170-175
Publisher name
Přírodovědecká fakulta Univerzity Karlovy
Place of publication
Praha
Event location
Praha
Event date
Sep 16, 2021
Type of event by nationality
EUR - Evropská akce
UT code for WoS article
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