Microfluidic device integrating single-cell extraction and electrical lysis for mass spectrometry detection of intracellular compounds
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68081715%3A_____%2F23%3A00556913" target="_blank" >RIV/68081715:_____/23:00556913 - isvavai.cz</a>
Alternative codes found
RIV/00216224:14310/23:00129965
Result on the web
<a href="http://hdl.handle.net/11104/0331026" target="_blank" >http://hdl.handle.net/11104/0331026</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1002/elps.202100379" target="_blank" >10.1002/elps.202100379</a>
Alternative languages
Result language
angličtina
Original language name
Microfluidic device integrating single-cell extraction and electrical lysis for mass spectrometry detection of intracellular compounds
Original language description
Analysis of cellular composition and metabolism at a single-cell resolution allows gaining more information about complex relationships of cells within tissues or whole living organisms by resolving the variance stemming from the cellular heterogeneity. Mass spectrometry (MS) is a perfect analytical tool satisfying the demanding requirements of detecting and identifying compounds present in such ultralow-volume samples of high chemical complexity. However, the method of sampling and sample ionization is crucial in obtaining relevant information. In this work, we present a microfluidic sampling platform that integrates single-cell extraction from MS-incompatible media with electrical cell lysis and nanoESI-MS analysis of human erythrocytes. Hemoglobin alpha and beta chains (300 amol/cell) were successfully identified in mass spectra of single-erythrocyte lysates.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
—
OECD FORD branch
10406 - Analytical chemistry
Result continuities
Project
<a href="/en/project/EF16_026%2F0008446" target="_blank" >EF16_026/0008446: Signal integration and epigenetic reprograming for plant productivity</a><br>
Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2023
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Electrophoresis
ISSN
0173-0835
e-ISSN
1522-2683
Volume of the periodical
44
Issue of the periodical within the volume
1-2
Country of publishing house
DE - GERMANY
Number of pages
10
Pages from-to
313-322
UT code for WoS article
000783231000001
EID of the result in the Scopus database
2-s2.0-85128334390