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An open source 3D printed autosampler for capillary electrophoresis

The result's identifiers

  • Result code in IS VaVaI

    <a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68081715%3A_____%2F23%3A00577529" target="_blank" >RIV/68081715:_____/23:00577529 - isvavai.cz</a>

  • Alternative codes found

    RIV/00216224:14740/23:00133474

  • Result on the web

    <a href="https://hdl.handle.net/11104/0346651" target="_blank" >https://hdl.handle.net/11104/0346651</a>

  • DOI - Digital Object Identifier

    <a href="http://dx.doi.org/10.1016/j.aca.2023.341832" target="_blank" >10.1016/j.aca.2023.341832</a>

Alternative languages

  • Result language

    angličtina

  • Original language name

    An open source 3D printed autosampler for capillary electrophoresis

  • Original language description

    In-house built capillary electrophoresis (CE) systems represent a significant share of laboratory instrumentation. In most of these instruments, sample injection is effected manually with low to moderate precision and requires skilled operators. Although few automated samplers have been previously developed, typically only one sample at a time can be injected. If a series of samples is to be analyzed, manual intervention is required. In the present work, we developed and constructed a fully automated, open source, CE autosampler, able to handle up to 14 different samples that can be used as a modular component of any in-house built CE instrument. Results: An inexpensive, 3D printed, open source, autosampler for CE was developed. The autosampler consists of two parts: an injection unit with carousel containing sample and electrolyte vials and a flushing unit, containing a miniature pressure/vacuum pump. The autosampler is operated by an Arduino Mega microcontroller and an Arduino code written in the laboratory. The injection sequence is entered through a keypad and LCD display by the user. The instrument can operate autonomously for extended periods of time. It was used for fully automated analysis and/or calibration of up to 14 samples with excellent injection repeatability reaching less than 2.7% RSD for peak areas. The sampler performance was tested with two independently built CE instruments, a CE system with contactless conductivity detection (C4D) and a CE system with laser induced fluorescence (LIF) detector.Significance and novelty: A novel, 3D printed, Arduino-based autosampler for CE was developed. The autosampler allows autonomous hydrodynamic injection of up to 14 different samples with fully programmable injection sequence, including capillary flushing and high voltage and data acquisition control. It provides the missing instrumental sampling setup for laboratory made CE instruments. It can be simply constructed based on the open -source blueprints in any laboratory and be a useful and time-saving add-on to any modular CE instrument.

  • Czech name

  • Czech description

Classification

  • Type

    J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database

  • CEP classification

  • OECD FORD branch

    10406 - Analytical chemistry

Result continuities

  • Project

    <a href="/en/project/GA22-23815S" target="_blank" >GA22-23815S: Metal nanoparticles as selective extractors of biological thiols from non-invasive fluid samples.</a><br>

  • Continuities

    I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Others

  • Publication year

    2023

  • Confidentiality

    S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Data specific for result type

  • Name of the periodical

    Analytica Chimica Acta

  • ISSN

    0003-2670

  • e-ISSN

    1873-4324

  • Volume of the periodical

    1279

  • Issue of the periodical within the volume

    OCT

  • Country of publishing house

    NL - THE KINGDOM OF THE NETHERLANDS

  • Number of pages

    8

  • Pages from-to

    341832

  • UT code for WoS article

    001084087000001

  • EID of the result in the Scopus database

    2-s2.0-85172254821