Investigation of interactions between biological thiols and gold nanoparticles by capillary electrophoresis with laser-induced fluorescence
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68081715%3A_____%2F24%3A00599201" target="_blank" >RIV/68081715:_____/24:00599201 - isvavai.cz</a>
Result on the web
<a href="https://analyticalsciencejournals.onlinelibrary.wiley.com/doi/epdf/10.1002/elps.202300248" target="_blank" >https://analyticalsciencejournals.onlinelibrary.wiley.com/doi/epdf/10.1002/elps.202300248</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1002/elps.202300248" target="_blank" >10.1002/elps.202300248</a>
Alternative languages
Result language
angličtina
Original language name
Investigation of interactions between biological thiols and gold nanoparticles by capillary electrophoresis with laser-induced fluorescence
Original language description
Biological thiols spontaneously form a stable Au-S dative bond with gold nanoparticles (AuNP) that might be used for their selective extraction and enrichment in biological samples. In this work, interactions of selected biological thiols (glutathione, cysteine, homocysteine [Hcys], cysteamine [CA], and N-acetylcysteine) with AuNP stabilized by different capping agents (citrate, Tween 20, Brij 35, CTAB, SDS) were investigated by UV-Vis spectroscopy and capillary electrophoresis with laser-induced fluorescence. Spectrophotometric measurements showed aggregation of Hcys and CA with AuNP. In contrast, it was confirmed by CE-LIF that biological thiols were adsorbed to all types of AuNP. Citrate-capped AuNP were selected for AuNP-based extraction of biological thiols from exhaled breath condensate (EBC). Dithiothreitol was utilized for desorption of biological thiols from the AuNP surface, which was followed by derivatization with eosin-5-maleimide and CE-LIF analysis. AuNP-based extraction increased the sensitivity of CE-LIF analysis, however, further optimization of methodology is necessary for accurate quantification of biological thiols in EBC.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10406 - Analytical chemistry
Result continuities
Project
<a href="/en/project/GA22-23815S" target="_blank" >GA22-23815S: Metal nanoparticles as selective extractors of biological thiols from non-invasive fluid samples.</a><br>
Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2024
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Electrophoresis
ISSN
0173-0835
e-ISSN
1522-2683
Volume of the periodical
45
Issue of the periodical within the volume
15-16
Country of publishing house
US - UNITED STATES
Number of pages
10
Pages from-to
1418-1427
UT code for WoS article
001176919600001
EID of the result in the Scopus database
2-s2.0-85181654053