Utilizing novel cationic phenylpyridine labeling in glycan profiling by capillary electrophoresis coupled to mass spectrometry
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68081715%3A_____%2F25%3A00602733" target="_blank" >RIV/68081715:_____/25:00602733 - isvavai.cz</a>
Alternative codes found
RIV/00216224:14160/25:00144257
Result on the web
<a href="https://hdl.handle.net/11104/0360018" target="_blank" >https://hdl.handle.net/11104/0360018</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1016/j.talanta.2024.127376" target="_blank" >10.1016/j.talanta.2024.127376</a>
Alternative languages
Result language
angličtina
Original language name
Utilizing novel cationic phenylpyridine labeling in glycan profiling by capillary electrophoresis coupled to mass spectrometry
Original language description
This work focuses on profiling N-linked glycans by capillary electrophoresis coupled to mass spectrometry using a novel fluorescent and mass spectrometry (MS) active derivatization tag. The label is based on 2-phenylpyridine bearing tertiary amine and hydrazide functionalities. It provides efficient labeling via hydrazone formation chemistry, promising fluorescence properties, and ionization efficiency in the positive ion MS mode. Electrophoretic analysis in a neutral-coated capillary allowed baseline separation of maltooligosaccharides with limits of detection in nanomolar concentrations. The developed labeling method was successfully applied to the analyses of N-linked glycans released from several glycoproteins such as bovine ribonuclease B, human immunoglobulin G, or chicken albumin.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10406 - Analytical chemistry
Result continuities
Project
<a href="/en/project/GA22-00236S" target="_blank" >GA22-00236S: Development of novel fluorescent labeling tags for electrophoretic analysis of glycoproteins associated with breast cancer</a><br>
Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2025
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Talanta
ISSN
0039-9140
e-ISSN
1873-3573
Volume of the periodical
285
Issue of the periodical within the volume
APR
Country of publishing house
NL - THE KINGDOM OF THE NETHERLANDS
Number of pages
6
Pages from-to
127376
UT code for WoS article
001389587800001
EID of the result in the Scopus database
2-s2.0-85211713110