Monolithic capillary columns based on methacryl-substituted polyhedral oligomeric silsesquioxane and sulfobetaine methacrylates synthesized in the wide-bore capillary: Evaluation and application in liquid chromatography
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68081715%3A_____%2F25%3A00618287" target="_blank" >RIV/68081715:_____/25:00618287 - isvavai.cz</a>
Result on the web
<a href="https://hdl.handle.net/11104/0365130" target="_blank" >https://hdl.handle.net/11104/0365130</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1016/j.microc.2025.113239" target="_blank" >10.1016/j.microc.2025.113239</a>
Alternative languages
Result language
angličtina
Original language name
Monolithic capillary columns based on methacryl-substituted polyhedral oligomeric silsesquioxane and sulfobetaine methacrylates synthesized in the wide-bore capillary: Evaluation and application in liquid chromatography
Original language description
The protocol for preparation of methacryl-substituted polyhedral oligomeric silsesquioxane-based monolithic stationary phases containing zwitterionic sulfobetaine monomers in 450 µm i.d. fused silica capillaries is presented. The monolithic columns were evaluated by scanning electron microscopy, inverse size-exclusion chromatography, and isocratic HPLC separation of model compounds. Nature of zwitterionic sulfobetaine monomers has distinct impact on the structure of the final monolithic material as well as on its polarity and separation ability. The columns are suitable for multimodal chromatography as confirmed by their capability to separate compounds over a wide range of polarities (alkylbenzenes, phenolics, aromatic carboxylic acids, a mixture of purine-, pyrimidine-bases, nucleosides, and 2-deoxynucleosides). The critical mobile phase composition for the transition from hydrophilic interaction liquid chromatography to reversed-phase mode is around 84 % of acetonitrile which confirms the prevailing non-polar nature of formed monoliths. The proposed preparation protocol allowed preparation of stable columns whose efficiency reached the minimal height of the theoretical plate 6–10 µm which decreased by less than 12 % during the two months of everyday use.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10406 - Analytical chemistry
Result continuities
Project
<a href="/en/project/GA23-04703S" target="_blank" >GA23-04703S: Alternative route to capillary monolithic silica columns from discrete particles treated with supercritical water</a><br>
Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2025
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Microchemical Journal
ISSN
0026-265X
e-ISSN
1095-9149
Volume of the periodical
212
Issue of the periodical within the volume
MAY
Country of publishing house
US - UNITED STATES
Number of pages
8
Pages from-to
113239
UT code for WoS article
001442833000001
EID of the result in the Scopus database
2-s2.0-85219746037