Bioconjugates of photon-upconversion nanoparticles with antibodies for the detection of prostate-specific antigen and p53 heterogeneous and homogeneous immunoassays

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68081715%3A_____%2F25%3A00618895" target="_blank" >RIV/68081715:_____/25:00618895 - isvavai.cz</a>

Result on the web

<a href="https://pubs.rsc.org/en/content/articlepdf/2025/nr/d5nr00176e" target="_blank" >https://pubs.rsc.org/en/content/articlepdf/2025/nr/d5nr00176e</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1039/D5NR00176E" target="_blank" >10.1039/D5NR00176E</a>

Result language

angličtina

Original language name

Bioconjugates of photon-upconversion nanoparticles with antibodies for the detection of prostate-specific antigen and p53 heterogeneous and homogeneous immunoassays

Original language description

Sensitive immunoassays for the detection of tumor biomarkers play an important role in early diagnosis and therapy of cancer. Using luminescent nanomaterials as labels can significantly improve immunoassay performance, especially in terms of sensitivity. Lanthanide-doped photon-upconversion nanoparticles (UCNPs) are nanocrystals capable of converting near-infrared radiation into visible light, and their emission spectra can be tuned by altering the dopant ions. In this study, the bioconjugation between UCNPs and biomolecules was optimized, and different conjugates of Er- and Tm-doped UCNPs (NaYF4:Yb3+,Er3+ and NaYF4:Yb3+,Tm3+) were prepared for detecting prostate-specific antigen (PSA) and tumor protein p53, comparing heterogeneous and homogeneous assay formats. The heterogeneous sandwich immunoassay achieved detection limits of 1.3 pg/mL of PSA and 330 pg/mL of p53. The homogeneous immunoassays were based on massively parallel spectroscopy (MPS), a novel artificial intelligence-aided single-molecule approach, utilizing conjugates of two different monoclonal antibodies with Er- and Tm-doped UCNPs, respectively. The conjugates provide distinct emission spectra, with only sandwich immunocomplexes of analyte and both types of labels showing both signals at the same location. The MPS-based immunoassay was suitable for observing the immunocomplexes in an aqueous dispersion using only a small sample volume. This innovative method achieved detection limits of 8.2 ng/mL and 390 pg/mL for PSA and p53, respectively. MPS eliminates the need for time-consuming washing steps required in heterogeneous immunoassays and is amenable to high-throughput applications.

Czech name

—

Czech description

—

Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

—

OECD FORD branch

10406 - Analytical chemistry

Project

<a href="/en/project/GA21-03156S" target="_blank" >GA21-03156S: Photon-upconversion labeling for microfluidic single-molecule immunoassays of protein biomarkers</a><br>

Continuities

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2025

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Nanoscale

ISSN

2040-3364

e-ISSN

2040-3372

Volume of the periodical

17

Issue of the periodical within the volume

18

Country of publishing house

GB - UNITED KINGDOM

Number of pages

12

Pages from-to

11401-11412

UT code for WoS article

001466645200001

EID of the result in the Scopus database

2-s2.0-105003275810