Bioconjugates of photon-upconversion nanoparticles with antibodies for the detection of prostate-specific antigen and p53 in heterogeneous and homogeneous immunoassays
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14310%2F25%3A00144317" target="_blank" >RIV/00216224:14310/25:00144317 - isvavai.cz</a>
Result on the web
<a href="https://pubs.rsc.org/en/content/articlelanding/2025/nr/D5NR00176E" target="_blank" >https://pubs.rsc.org/en/content/articlelanding/2025/nr/D5NR00176E</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1039/D5NR00176E" target="_blank" >10.1039/D5NR00176E</a>
Alternative languages
Result language
angličtina
Original language name
Bioconjugates of photon-upconversion nanoparticles with antibodies for the detection of prostate-specific antigen and p53 in heterogeneous and homogeneous immunoassays
Original language description
Sensitive immunoassays for the detection of tumor biomarkers play an important role in the early diagnosis and therapy of cancer. Using luminescent nanomaterials as labels can significantly improve immunoassay performance, especially in terms of sensitivity. Lanthanide-doped photon-upconversion nanoparticles (UCNPs) are nanocrystals capable of converting near-infrared radiation into visible light, and their emission spectra can be tuned by altering the dopant ions. In this study, the bioconjugation between UCNPs and biomolecules was optimized, and different conjugates of Er- and Tm-doped UCNPs (NaYF4:Yb3+,Er3+ and NaYF4:Yb3+,Tm3+) were prepared for detecting prostate-specific antigen (PSA) and tumor protein p53, comparing heterogeneous and homogeneous assay formats. The heterogeneous sandwich immunoassay achieved detection limits of 1.3 pg mL-1 for PSA and 330 pg mL-1 for p53. The homogeneous immunoassays were based on massively parallel spectroscopy (MPS), a novel artificial intelligence-aided single-molecule approach, utilizing conjugates of two different monoclonal antibodies with Er- and Tm-doped UCNPs, respectively. The conjugates provide distinct emission spectra, with only sandwich immunocomplexes of analytes and both types of labels showing signals at the same location. MPS was suitable for observing the immunocomplexes in an aqueous dispersion using only a small sample volume. This innovative method achieved detection limits of 8.2 ng mL-1 and 390 pg mL-1 for PSA and p53, respectively. MPS eliminates the need for time-consuming washing steps required in heterogeneous immunoassays and is amenable to high-throughput applications.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10406 - Analytical chemistry
Result continuities
Project
<a href="/en/project/GA21-03156S" target="_blank" >GA21-03156S: Photon-upconversion labeling for microfluidic single-molecule immunoassays of protein biomarkers</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Others
Publication year
2025
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Nanoscale
ISSN
2040-3364
e-ISSN
2040-3372
Volume of the periodical
17
Issue of the periodical within the volume
18
Country of publishing house
GB - UNITED KINGDOM
Number of pages
12
Pages from-to
11401-11412
UT code for WoS article
001466645200001
EID of the result in the Scopus database
2-s2.0-105003275810