Structure elucidation of multicolor emissive graphene quantum dots towards cell guidance

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68081723%3A_____%2F22%3A00553531" target="_blank" >RIV/68081723:_____/22:00553531 - isvavai.cz</a>

Alternative codes found

RIV/00216224:14310/22:00119559 RIV/00216305:26620/22:PU147301

Result on the web

<a href="https://pubs.rsc.org/en/content/articlelanding/2022/QM/D1QM01126J" target="_blank" >https://pubs.rsc.org/en/content/articlelanding/2022/QM/D1QM01126J</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1039/d1qm01126j" target="_blank" >10.1039/d1qm01126j</a>

Result language

angličtina

Original language name

Structure elucidation of multicolor emissive graphene quantum dots towards cell guidance

Original language description

Graphene quantum dots (GQDs) can become excellent bioimaging tools when tuned to emit at larger wavelengths due to the minimal tissue absorbance and emission in this range. Tuning the GQD structure can help but understanding the chemical structure responsible for their properties remains challenging. Herein, we elucidated the structure of GQDs synthesized from glucose and ammonium hydroxide using a fast microwave-assisted hydrothermal protocol. Remarkably, these GQDs exhibited emission from the NUV-Vis up to the NIR range. The structure and chemical composition were elucidated using advanced NMR techniques, such as two-dimensional nuclear magnetic resonance, combined with traditional spectroscopy and electron microscopy. The graphitic core composed of pyrazines presented localized defects and lower rotation mobility compared with their edges that were mainly formed by hydroxyl, acid, and amine functional groups, which paved the way for the observed multicolor red-shifted fluorescence emission. Confocal laser scanning microscopy revealed functional cell imaging in a wide spectral range of fluorescence from bright purple to red, confirming the uptake of GQDs by the cells without any observable toxicity. The non-cytotoxicity was further proved by the chemiluminescence cell viability adenosine triphosphate (ATP) assay. Combined with the tunable GQD emission, it gives them the potential to act as bioimaging carriers starting a new phase for their use in vivo.

Czech name

—

Czech description

—

Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

—

OECD FORD branch

21002 - Nano-processes (applications on nano-scale); (biomaterials to be 2.9)

Project

Result was created during the realization of more than one project. More information in the Projects tab.

Continuities

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2022

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Materials Chemistry Frontiers

ISSN

2052-1537

e-ISSN

2052-1537

Volume of the periodical

6

Issue of the periodical within the volume

2

Country of publishing house

GB - UNITED KINGDOM

Number of pages

9

Pages from-to

145-154

UT code for WoS article

000730146400001

EID of the result in the Scopus database

2-s2.0-85123925022