A Versatile High-Vacuum Cryo-transfer System for Cryo-microscopy and Analytics

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68081731%3A_____%2F16%3A00465045" target="_blank" >RIV/68081731:_____/16:00465045 - isvavai.cz</a>
Result on the web
<a href="http://dx.doi.org/10.1016/j.bpj.2016.01.024" target="_blank" >http://dx.doi.org/10.1016/j.bpj.2016.01.024</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1016/j.bpj.2016.01.024" target="_blank" >10.1016/j.bpj.2016.01.024</a>

Result language
angličtina
Original language name
A Versatile High-Vacuum Cryo-transfer System for Cryo-microscopy and Analytics
Original language description
Cryogenic microscopy methods have gained increasing popularity, as they offer an unaltered view on the architecture of biological specimens. As a prerequisite, samples must be handled under cryogenic conditions below their recrystallization temperature, and contamination during sample transfer and handling must be prevented. We present a high-vacuum cryo-transfer system that streamlines the entire handling of frozen-hydrated samples from the vitrification process to low temperature imaging for scanning transmission electron microscopy and transmission electron microscopy. A template for cryo-electron microscopy and multimodal cryo-imaging approaches with numerous sample transfer steps is presented.
Czech name
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Czech description
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Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
JA - Electronics and optoelectronics
OECD FORD branch
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Project
<a href="/en/project/GA14-20012S" target="_blank" >GA14-20012S: Quantitative imaging in SEM with elastically scattered electrons</a><br>
Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year
2016
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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