A versatile high-vacuum cryo transfer system for cryo microscopy and analytics
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68081731%3A_____%2F16%3A00465341" target="_blank" >RIV/68081731:_____/16:00465341 - isvavai.cz</a>
Result on the web
<a href="http://dx.doi.org/10.1002/9783527808465.EMC2016.6577" target="_blank" >http://dx.doi.org/10.1002/9783527808465.EMC2016.6577</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1002/9783527808465.EMC2016.6577" target="_blank" >10.1002/9783527808465.EMC2016.6577</a>
Alternative languages
Result language
angličtina
Original language name
A versatile high-vacuum cryo transfer system for cryo microscopy and analytics
Original language description
The conservation of the native state during sample preparation is mandatory for a correct interpretation of any micrograph. Particularly for EM, the preservation of the pristine architecture is challenging. If not imaged in situ, two different strategies can be followed to prepare hydrated samples for electron microscopy: the conventional and the cryogenic routine. Conventional preparation protocols typically rely on the chemical fixation and staining of the sample material, whereby both steps are known to induce artefacts. The second preparation routine, introduced by Moor and Dubochet, circumvents chemical artefacts. The basic principle of every cryogenic (cryo) preparation protocol is the physical immobilization of the sample in a frozen-hydrated solid state, by vitrifying the sample within milliseconds. Due to the excellent structural preservation cryo imaging techniques have gained increasing popularity.
Czech name
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Czech description
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Classification
Type
D - Article in proceedings
CEP classification
JA - Electronics and optoelectronics
OECD FORD branch
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Result continuities
Project
<a href="/en/project/GA14-20012S" target="_blank" >GA14-20012S: Quantitative imaging in SEM with elastically scattered electrons</a><br>
Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2016
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Article name in the collection
EMC2016. The 16th European Microscopy Congres. Proceedings
ISBN
9783527808465
ISSN
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e-ISSN
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Number of pages
2
Pages from-to
412-413
Publisher name
Wiley
Place of publication
Oxford
Event location
Lyon
Event date
Aug 28, 2016
Type of event by nationality
WRD - Celosvětová akce
UT code for WoS article
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