Processing a Biological Tissue from Cryo-SEM to Replica

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68081731%3A_____%2F16%3A00465204" target="_blank" >RIV/68081731:_____/16:00465204 - isvavai.cz</a>

Result on the web

<a href="http://dx.doi.org/10.1017/S1431927616002038" target="_blank" >http://dx.doi.org/10.1017/S1431927616002038</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1017/S1431927616002038" target="_blank" >10.1017/S1431927616002038</a>

Result language

angličtina

Original language name

Processing a Biological Tissue from Cryo-SEM to Replica

Original language description

Cryo-SEM is a technique suitable for observation of frozen biological samples. The cryo-preparation of tissue fragments without using cryo-protectants can destroy intracellular compartments and cytoplasm. On the other hand the use of cryoprotectants can prevent deeper etching of the medium and thus it often leads to more complicated detection of cell membranes, which are important for orientation in the sample during its observation. This can be managed by shadowing of the fractured structures by platinum and it may help to heighten the structures as is done in production of replicas which can give more detail at higher magnification. Here we report how the replicas can be prepared in the device ACE600 (Leica microsystems) equipped with the cryo-attachement and two guns for metal sputtering and carbon evaporation, and the combination of cryo-SEM and replicas.

Czech name

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Czech description

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Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

JA - Electronics and optoelectronics

OECD FORD branch

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Project

Result was created during the realization of more than one project. More information in the Projects tab.

Continuities

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2016

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Microscopy and Microanalysis

ISSN

1431-9276

e-ISSN

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Volume of the periodical

22

Issue of the periodical within the volume

S3

Country of publishing house

US - UNITED STATES

Number of pages

2

Pages from-to

236-237

UT code for WoS article

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EID of the result in the Scopus database

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