Is Sputtering Sufficient for Production of Replicas?

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68081731%3A_____%2F16%3A00465205" target="_blank" >RIV/68081731:_____/16:00465205 - isvavai.cz</a>

Result on the web

<a href="http://dx.doi.org/10.1017/S1431927616001070" target="_blank" >http://dx.doi.org/10.1017/S1431927616001070</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1017/S1431927616001070" target="_blank" >10.1017/S1431927616001070</a>

Result language

angličtina

Original language name

Is Sputtering Sufficient for Production of Replicas?

Original language description

In the production of replicas there are many parameters which influence the authenticity and precision of the biological structures, e.g. manipulation with tissue pieces or cells, conditions of freeze-fracture technique or the process of replica preparation alone. The original protocols of replica processing have employed for many years devices such as BAF060 (BAL-TEC) that is equipped by cryo-stage and two guns for well specified directional metal and carbon evaporation. This technology is relatively expensive, in comparison to modern devices for cryo-preparation for electron microscopy like ACE600 (Leica microsystems). However, these modern devices are usually based on the metal sputtering in thenargon atmosphere and evaporation of carbon (e.g. ACE600 or the precursor MED020). Here we report on the protocol for production replicas using both types of devices (ACE600 and BAF060) andncomparison of the results.

Czech name

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Czech description

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Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

JA - Electronics and optoelectronics

OECD FORD branch

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Project

Result was created during the realization of more than one project. More information in the Projects tab.

Continuities

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2016

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Microscopy and Microanalysis

ISSN

1431-9276

e-ISSN

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Volume of the periodical

22

Issue of the periodical within the volume

S3

Country of publishing house

US - UNITED STATES

Number of pages

2

Pages from-to

44-45

UT code for WoS article

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EID of the result in the Scopus database

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