Coherent anti-Stokes Raman scattering microscopy through a multimode fiber endoscope

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68081731%3A_____%2F20%3A00535569" target="_blank" >RIV/68081731:_____/20:00535569 - isvavai.cz</a>

Result on the web

<a href="https://www.spiedigitallibrary.org/conference-proceedings-of-spie/11359/1135907/Coherent-anti-Stokes-Raman-scattering-microscopy-through-a-multimode-fiber/10.1117/12.2555080.short" target="_blank" >https://www.spiedigitallibrary.org/conference-proceedings-of-spie/11359/1135907/Coherent-anti-Stokes-Raman-scattering-microscopy-through-a-multimode-fiber/10.1117/12.2555080.short</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1117/12.2555080" target="_blank" >10.1117/12.2555080</a>

Result language

angličtina

Original language name

Coherent anti-Stokes Raman scattering microscopy through a multimode fiber endoscope

Original language description

Advanced wavefront-shaping methods can be used to transform a simple multimode fiber into an ultra-thin laser scanning microscope. Here, we extend this technique to label-free non-linear microscopy with chemical contrast using coherent anti-Stokes Raman scattering (CARS) through a multimode fiber endoscope, which opens up new avenues for instant and in-situ diagnosis of potentially malignant tissue. We use a commercial, 125 μm diameter, 0.29 NA, GRIN fiber as the endoscopic probe. Wavefront shaping on a spatial light modulator is used to create a focus, where the 1-2 ps long pump and Stokes pulses are overlapped in time, which is scanned behind the fiber facet across the sample. The chemical selectivity is demonstrated by imaging 2 μm polystyrene and 2.5 μm PMMA beads with per pixel integration time as low as 1 ms for epi-detection. Epi-detection through the fiber is possible despite the fact that the CARS signal is emitted mainly in the forward direction, away from the fiber facet. Detecting the back-scattered signal from the underlying tissue, requires a large detector aperture to be efficient. By detecting through both the core and the cladding of the fiber, we obtain sufficient detection efficiency. © COPYRIGHT SPIE. Downloading of the abstract is permitted for personal use only.

Czech name

—

Czech description

—

Type

D - Article in proceedings

CEP classification

—

OECD FORD branch

10306 - Optics (including laser optics and quantum optics)

Project

Result was created during the realization of more than one project. More information in the Projects tab.

Continuities

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2020

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Article name in the collection

Biomedical Spectroscopy, Microscopy, and Imaging 2020. Proceedings of SPIE

ISBN

978-151063490-9

ISSN

0277-786X

e-ISSN

—

Number of pages

9

Pages from-to

162267

Publisher name

SPIE

Place of publication

Bellingham

Event location

France

Event date

Apr 6, 2020

Type of event by nationality

WRD - Celosvětová akce

UT code for WoS article

000674749600004