Correlative light-environmental scanning electron microscopy of plasma membrane efflux carriers of plant hormone auxin
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68081731%3A_____%2F21%3A00546239" target="_blank" >RIV/68081731:_____/21:00546239 - isvavai.cz</a>
Alternative codes found
RIV/00216208:11310/21:10438235
Result on the web
<a href="https://www.mdpi.com/2218-273X/11/10/1407" target="_blank" >https://www.mdpi.com/2218-273X/11/10/1407</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.3390/biom11101407" target="_blank" >10.3390/biom11101407</a>
Alternative languages
Result language
angličtina
Original language name
Correlative light-environmental scanning electron microscopy of plasma membrane efflux carriers of plant hormone auxin
Original language description
Fluorescence light microscopy provided convincing evidence for the domain organization of plant plasma membrane (PM) proteins. Both peripheral and integral PM proteins show an inhomo-geneous distribution within the PM. However, the size of PM nanodomains and protein clusters is too small to accurately determine their dimensions and nano-organization using routine confocal fluorescence microscopy and super-resolution methods. To overcome this limitation, we have developed a novel correlative light electron microscopy method (CLEM) using total internal reflection fluorescence microscopy (TIRFM) and advanced environmental scanning electron microscopy (A-ESEM). Using this technique, we determined the number of auxin efflux carriers from the PINFORMED (PIN) family (NtPIN3b-GFP) within PM nanodomains of tobacco cell PM ghosts. Protoplasts were attached to coverslips and immunostained with anti-GFP primary antibody and secondary antibody conjugated to fluorochrome and gold nanoparticles. After imaging the nanodomains within the PM with TIRFM, the samples were imaged with A-ESEM without further processing, and quantification of the average number of molecules within the nanodomain was performed. Without requiring any post-fixation and coating procedures, this method allows to study details of the organization of auxin carriers and other plant PM proteins.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10611 - Plant sciences, botany
Result continuities
Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2021
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Biomolecules
ISSN
2218-273X
e-ISSN
2218-273X
Volume of the periodical
11
Issue of the periodical within the volume
10
Country of publishing house
CH - SWITZERLAND
Number of pages
13
Pages from-to
1407
UT code for WoS article
000712762200001
EID of the result in the Scopus database
2-s2.0-85115616208