Analysis of GFP and AHP5-GFP Fusion Protein in the Plant Extract Using CE-LIF

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14740%2F12%3A00057866" target="_blank" >RIV/00216224:14740/12:00057866 - isvavai.cz</a>
Result on the web
<a href="http://www.ce-ce.org/cece2012/Proceedings%20CECE%202012%20Final+late%20abstracts_web.pdf" target="_blank" >http://www.ce-ce.org/cece2012/Proceedings%20CECE%202012%20Final+late%20abstracts_web.pdf</a>
DOI - Digital Object Identifier
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Result language
angličtina
Original language name
Analysis of GFP and AHP5-GFP Fusion Protein in the Plant Extract Using CE-LIF
Original language description
We focused on the possibility of determination green fluorescent protein (GFP) and Arabidopsis histidine phophotransfer protein 5 fused with GFP (AHP5-GFP) in complex plant samples using capillary electrophoresis with laser-induced fluorescence detection(CE-LIF). The control method is western blotting, where the anti-GFP antibodies enable monitoring the expression level of AHP5-GFP in individual organisms. It was found that CE-LIF is sensitive enough for both proteins. In the future, the developed methods should be used to analyze the phosphorylated and unphosphorylated AHP5-GFP.
Czech name
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Czech description
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Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>Z - Vyzkumny zamer (s odkazem do CEZ)<br>S - Specificky vyzkum na vysokych skolach

Publication year
2012
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Article name in the collection
CECE 2012 9th International Interdisciplinary Meeting on Bioanalysis
ISBN
9788090495913
ISSN
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e-ISSN
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Number of pages
4
Pages from-to
186-189
Publisher name
9th International Interdisciplinary Meeting on Bioanalysis, CECE 2012
Place of publication
Brno
Event location
Brno
Event date
Jan 1, 2012
Type of event by nationality
WRD - Celosvětová akce
UT code for WoS article
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